益气养阴方对Lewis肺癌移植小鼠肿瘤生长影响的实验研究  被引量:1

Effects of Yiqi Yangyin Decoction on Tumor Growth of Suppressing Tumor-bearing Lewis Lung Cancer Mice

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作  者:杨国良[1] 胡丹丹[2] YANG Guoliang HU Dandan(Integrated Chinese and Western Medicine Hospital of Zhejiang Province,Hangzhou 310004,China The Third Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310053 ,China)

机构地区:[1]浙江省中西医结合医院,浙江杭州310004 [2]浙江中医药大学附属第三医院,浙江杭州310053

出  处:《山东中医药大学学报》2017年第3期273-276,284,共5页Journal of Shandong University of Traditional Chinese Medicine

基  金:浙江省自然基金资助项目(编号:LQ12H27006);浙江省中医药科技计划资助项目(编号:2016ZA108);浙江省中医药科技计划项目(编号:2015ZB084)

摘  要:目的:通过不同剂量益气养阴方对Lewis肺癌移植小鼠肿瘤生长及肿瘤组织中Notch信号表达、CD4^+CD25^+Treg细胞比例及细胞因子IL-10、TNF-β表达影响的观察,探讨益气养阴方干预肺癌的量效关系及机制。方法:将60只近交系C57BL/6纯系小鼠行Lewis肺癌细胞移植造模后随机分为4组:模型组、化疗组、益气养阴方等效剂量组、益气养阴方大剂量组。模型组仅行造模;等效剂量组、大剂量组按小鼠体质量灌胃给药,每天3次;化疗组以CTX腹腔注射化疗,每周一次。记录各组小鼠瘤体大小,观察期满后留取标本行HE染色及Notch信号表达检测。结果:(1)肿瘤相对体积(RTV)及肿瘤相对抑制率:药物干预组RTV值均较模型组缩小(P<0.01),组间比较大剂量组RTV均低于其他三组(P<0.01),而化疗组RTV低于等效剂量组(P<0.05);大剂量组肿瘤抑制率高于其他三组(P<0.05);(2)肿瘤组织中Notch信号通路表达及CD4^+CD25^+Treg细胞:等效剂量组、高剂量组、化疗组Notch1mRNA表达、CD4^+CD25^+Treg均较模型组降低(P<0.05),等效剂量组Notch1mRNA表达高于化疗组(P<0.05);高剂量组较等效剂量组及化疗组Notch1mRNA表达量低(P<0.05);等效剂量组、益气养阴高剂量组、化疗组Notch3mRNA表达均较模型组降低(P<0.05),但等效剂量组Notch3mRNA表达与化疗组比较差异无统计学意义(P>0.05);高剂量组较等效剂量组及化疗组Notch3mRNA表达量降低(P<0.05);等效剂量组、高剂量组、化疗组Jagged1mRNA及HeslmRNA表达均较模型组降低(P<0.05),等效剂量组与化疗组Jagged1mRNA及HeslmRNA表达量降低,差异具有统计学意义(P<0.05);高剂量组较等效剂量组及化疗组Jagged1mRNA及HeslmRNA表达量降低(P<0.05);(3)肿瘤组织中IL-10、TNF-β表达:药物干预各组肿瘤组织中IL-10、TNF-β表达均较模型组降低(P<0.05)。药物干预组间比较:化疗组IL-10、TNF-β表达降低最明显,其次为高剂量组。结论:益气养阴方有抑制Lewis�Objective:To investigate the relationship between the different dose and the effects of Yiqi Yangyin decoction on suppressing tumor-bearing Lewis lung cancer mice and discuss the mechanism through the Notch signal pathway,CD4+CD25+Treg and IL-10,TNF-β. Methods:Total 60 inbred C57BL/6 mice bearing Lewis lung cancer were randomly divided into four groups:the model group(group A),chemotherapy group(group B),equivalent dose group of Yiqi Yangyin decoction(group C) and high dose group of Yiqi Yangyin decoction(group D). The mice in group A were only models. The mice in group B were treated byintraperitoneal injection of 20 mg/kg CTX every week. The mice in group C were treated with Yiqi Yangyin decoction of 0.345 mL/g by gastric lavage. The mice in group D were treated with Yiqi Yangyin decoction of 1.36 mL/g by gastric lavage. All mice were treated for 30 days and fed with commercial diet,at the same time the tumor volume was recorded. Then all the mice were sacrificedand all tumor tissues were made HE sections routinely,meanwhile the Notch signal expression in tumor tissues was tested. Resuhs:①Relative tumor volume(RTV) and relative suppressing-tumor rate. RTVs of group B,C,D were reduced more than that of group A(P〈0.01). RTV of group D was reduced mostly than those of other three groups(P〈0.01). RTV of group B was reduced more than that of group C(P〈0.05). The relative suppressing-tumor rate of group D was highest in the four groups(P〈0.05). ②The expressions of Notch signal pathway and CD4+CD25+Treg:the expressions of NotchlmRNA and CD4+CD25+Treg in group B,C,D were lower than those in group A(P〈0.05). The expressions of NotchlmRNA and CD4+CD25+Treg in group C were higher than those in group B(P〈0.05),but the expressions of NotchlmRNA and CD4+CD25+ Treg in group D were lowest in group B,C(P〈0.05). The changes of the expressions of JaggedlmRNA, HeslmRNA and Notch3mRNA were similar with the changes of NotchlmRNA in the f

关 键 词:益气养阴方 NOTCH信号 肺癌 CD4+CD25+TREG细胞 IL-10 TNF-Β 量效关系 小鼠 

分 类 号:R285.5[医药卫生—中药学]

 

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