钙离子与肥大细胞在针刺效应产生中作用的研究  

Study on the Interaction of Ca^(2+) and Mast cells in Acupuncture Effect

作　　者：裴莹[1] 赵雪[2] 李忠正[2] 郭义[2] 

机构地区：[1]辽宁中医药大学附属医院神经内科,辽宁沈阳110032 [2]天津中医药大学实验针灸学研究中心,天津300193

出　　处：《临床和实验医学杂志》2017年第12期1145-1148,共4页Journal of Clinical and Experimental Medicine

基　　金：国家自然科学基金资助项目(81330088)

摘　　要：目的在前期研究肯定钙离子(Ca^(2+))和肥大细胞在针刺效应产生中具有重要作用的基础上,观察络合足三里穴区Ca^(2+)后,穴区肥大细胞的形态学变化,研究在针效产生中钙离子与肥大细胞两者相互作用的关系,揭示针效产生的始动机制,为研究提供可靠的实验依据。方法复制小鼠急性胃损伤模型,将不同浓度的乙二醇双乙胺醚四乙酸(EGTA)进行穴位注射,同时将足三里穴区的Ca^(2+)进行络合,采用光镜进行观察,主要观察足三里穴区皮肤、肌肉等部位的肥大细胞数目、脱颗粒的肥大细胞数目等,计算肥大细胞脱颗粒率。结果机体在胃损伤状态下,针刺能够促进足三里穴位周围的肥大细胞脱颗粒。络合足三里穴区Ca^(2+)后,针刺不能对机体的穴区皮肤、肌肉处的肥大细胞脱颗粒。不同浓度的EGTA穴位注射络合穴区Ca^(2+)时,EGTA浓度越高,针刺后机体穴区皮肤、肌肉处脱颗粒的肥大细胞数量越少,同时肥大细胞脱颗粒率越低,反之亦然。结论针刺效应产生过程中,钙离子与肥大细胞具有一定的作用关系。Objective To observe the morphological change of mast cells and acupuncture before and after complexing Ca2＋ of Zusanli （ST 36） , study on the interaction of Ca2 ＋ and mast cells, provide the experimental evidence of initial mechanism of acupuncture effect. Methods Hydrochloric acid was used to establish the acute gastric injury model in mice. Gastric emptying rate was applied to evaluate the improvement of gastric function by needling ST36. Different concentrations of EGTA were injected into ST36 respectively and the amount of mast cells, degranulat-ed mast cells in skin and muscle tissues of ST36 were counted by microscope. Results Acupuncture can promote the degranulation of mast cells in gastric injury mice. Degranulation of mast cells can be restrained by complexing the Ca2＋ of ST36. Theconcentration of Ca2 ＋ has the positive relationship with the degranulation rate of mast cells. Conclusion Ca2 ＋ and mast cells have close relationship and both of them take part in acu-puncture effect.

关 键 词：小鼠 针刺 钙离子 肥大细胞 

分 类 号：R245[医药卫生—针灸推拿学]