GDF11在棕榈酸诱导骨骼肌细胞胰岛素抵抗中的作用研究  

Role of GDF11 in skeletal muscle cell insulin resistance induced by palmitate

作　　者：敬媛媛[1] 吴凡[1] 李蓉[1] 

机构地区：[1]重庆医科大学附属第一医院内分泌科,重庆400016

出　　处：《中国药理学通报》2017年第6期767-771,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81200588);国家临床重点专科建设项目(No 2011-170)

摘　　要：目的探讨GDF11对棕榈酸诱导骨骼肌细胞胰岛素抵抗的影响。方法用棕榈酸构建骨骼肌细胞胰岛素抵抗模型,分为对照组、GDF11干预组、棕榈酸干预组和GDF11联合棕榈酸干预组。CCK-8检测细胞活力,2NBDG检测细胞葡萄糖摄取。实时荧光定量PCR检测肌管标志基因(desmin、myogenin),胰岛素介导葡萄糖摄取相关基因(GLUT-4、IRS-1)及PGC-1α的表达。Western blot检测PGC-1α蛋白水平的表达。结果不同浓度GDF11对骨骼肌细胞活力无明显影响。与对照组相比,棕榈酸干预组葡萄糖摄取及GLUT-4、IRS-1、PGC-1α的表达明显降低(P<0.05)。与棕榈酸干预组相比,GDF11联合棕榈酸干预组葡萄糖摄取及GLUT-4、IRS-1、PGC-1α的表达无明显变化。结论棕榈酸可成功诱导骨骼肌细胞胰岛素抵抗,而GDF11对骨骼肌细胞胰岛素抵抗没有明显改善作用。Aim To investigate the role of GDF11 in palmitate induced skeletal muscle insulin resistance. Methods The C2C12 cells were sorted into control group, GDF11 intervention group, palmitate group and GDF11 combined with palmitate group. Cell viability was measured by CCK-8, and the glucose uptake was determined by 2NBDG. The mRNA level of myotube marker genes （ desmin, myogenin ） , insulin mediate glucose uptake related genes （ GLUT-4, IRS-1 ） and PGC-1α were tested by RT-PCR. The protein expres-sion of PGC-1α was detected by western blot. Results GDF11 had little effect on cell viability of skeletalmuscle cells. Compared with control group, the glu-cose uptake and the expression of GLUT-4, IRS-1, PGC-1α were significantly decreased by palmitate in-tervention. Compared with palmitate group, the glu-cose uptake and the expression of GLUT-4, IRS-1, PGC-1α were not significantly changed by GDF11. Conclusion Palmitate can induce skeletal muscle cell insulin resistance, but GDF11 may not significantly improve the skeletal muscle cell insulin resistance.

关 键 词：GDF11 骨骼肌细胞 胰岛素抵抗 PGC-1Α 棕榈酸 葡萄糖摄取 

分 类 号：R322.74[医药卫生—人体解剖和组织胚胎学] R329.24[医药卫生—基础医学]