CXCR4基因转染对骨髓间充质干细胞体外生物学行为的影响  

Effects of CXCR4 gene transfection on biological behavior of bone marrow mesenchymal stem cells(in vitro)

作　　者：王玉莹[1] 张囡[1] 李秀丽[1] 王雅萌[1] 李少恒[1] 闫宇辉[1] 宋捷[1] 杨静娴[1] 闻庆平[2] 

机构地区：[1]辽宁中医药大学药学院药理学教研室,辽宁大连116600 [2]大连医科大学,辽宁大连116044

出　　处：《中国药理学通报》2017年第6期806-813,共8页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81273923)

摘　　要：目的观察对转染CXCR4基因的骨髓间充质干细胞体外生物学行为的影响。方法将培养的骨髓间充质干细胞分为3组,GFP组、CXCR4^+组、CXCR4^-组,转染趋化因子受体CXCR4,并用免疫荧光细胞化学法、流式细胞仪法及Transwell小室细胞趋化实验,体外研究了CXCR4高表达及低表达对MSCs增殖、分化与迁移能力的影响。结果 CXCR4高表达及低表达均不影响MSCs的增殖能力,对其向肺组织分化的能力也没有影响。与GFP-MSCs组相比,CXCR4^+-MSCs组迁移的细胞数量明显升高,而CXCR4^--MSCs组迁移细胞数量差异无显著性。结论 CXCR4高表达及低表达不改变MSCs的增殖、分化能力,然而CXCR4高表达明显增强MSCs向炎症病灶的迁移能力。说明CXCR4高表达的MSCs移植入体后将会更快速、更大量地到达病变区域参与组织修复,明显增强疗效。Aim To observate the effect of chemokinereceptor( CXCR 4) gene transfection on biologicalbehavior of bone marrow mesenchymal stem cells in vitro. Methods Firstly,bone marrow mesenchymal stem cells were divided into three groups: GFP( transfected GFP into MSCs),CXCR4^+( transfected CXCR4^+into MSCs) and CXCR4^-( transfected CXCR4^-into MSCs) group. Then,their capacity of proliferation,differentiation and migration ability( in vitro)was assessed with immunofluorescence cytochemistry method,flow cytometry assay and Transwell cell chemotaxis test. Results The high or low expression of CXCR4 had no effect on their ability of proliferation and differentiation into lung tissue. Compared with GFP group, however, CXCR4^+-MSCs group significantly increased the number of migrating cells,while CX-CR4^--MSCs group showed no change in the number of migrating cells. Conclusions The proliferation and differentiation capacities are not affected by the high or low expression of CXCR4. The high expression of CXCR4 can significantly enhance the migration ability of MSCs to inflammatory lesions,and the low one has no effect on the migration of the cells. After the transplantation of MSCs,CXCR4' s high expression will access to the lesion area to participate in tissue repairing rapidly and largely,significantly enhancing the therapeutic efficacy.

关 键 词：骨髓间充质干细胞 趋化因子受体CXCR4 基因转染 增殖 分化 迁移 

分 类 号：R322.35[医药卫生—人体解剖和组织胚胎学] R329.24[医药卫生—基础医学]