绿孔雀粪便细菌多样性及其酶活性研究  

Diversity and Enzymatic Activities Analysis of Bacteria Isolated from Pavomuticus Feces

作　　者：曹艳茹[1,2] 孔德军[1,2] 李有龙 刘建惠[1,2] 刘永张 师蕾 王定康[5] 

机构地区：[1]昆明学院生命科学与技术系,云南昆明650214 [2]云南省高校特色生物资源开发与利用重点实验室,云南昆明650214 [3]云南野生动物园,云南昆明650218 [4]圆通山动物园,云南昆明650000 [5]昆明学院农学院,云南昆明650214

出　　处：《昆明学院学报》2017年第3期75-78,94,共5页Journal of Kunming University

基　　金：云南省应用基础研究计划资助项目(2013FZ096);昆明学院云南省高校特色生物资源开发与利用重点实验室开放基金资助项目(GXK2015);云南省高校优势特色重点学科(生态学)建设项目资助;国家自然科学基金项目(31300012;31660002);云南省生物多样性保护专项资金项目(Y3038891121)

摘　　要：以绿孔雀粪便为研究对象,通过纯培养的方法研究细菌的数量、种类.结果表明,分离的25株菌分别为,小单孢菌属(Micromonospora)、棒状杆菌属(Corynebacterium)、芽孢八叠球菌属(Sporosarcina)、微球菌属(Micrococcus)、节杆菌属(Arthrobacter)、异常球菌属(Deinococcus)和巴尔加瓦属(Bhargavaea).检测分离菌株的4种酶活,淀粉酶呈阳性的菌株有8株,纤维素酶有11株,蛋白酶有14株,脂肪酶有10株.这些阳性菌株可为微生物酶资源的开发应用提供材料,也可为认识绿孔雀肠道微生物的功能提供数据支持.Using Pavomuticu＇s feces as the object of study, we studied the amount and sorts of fecal Bacteria with culture-dependent approach. The result showed that the twenty-five representative isolates were Micromonospora, Corynebacterium, Sporosarcina, Micrococcus, Arthrobacter, Deinococcus and Bhargavaea. Four enzyme activities were examined from the isolated bacterial stains among which 8 stains were positive for amylase; 11 strains were positive for cellulose; 14 strains were positive for protease and 10 strains were positive for lipase. The positive stains enrich potential resource used for Bacteria development and utilization, and offer data for inferring the function of fecal microbes.

关 键 词：绿孔雀粪便 细菌 多样性 酶活 

分 类 号：Q939[生物学—微生物学] S858[农业科学—临床兽医学]