葛花总黄酮对大鼠心肌缺血再灌注动物损伤模型的保护作用  

Protection of Total Flavonoids of Puerariae Lobatae Flos on Myocardial Ischemia-reperfusion Injury in Rats

作　　者：范鹤馨 张志强[2] 

机构地区：[1]湘雅博爱康复医院,长沙410000 [2]河南中医药大学,郑州450046

出　　处：《中国实验方剂学杂志》2017年第12期119-125,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家临床重点专科建设项目经费项目(国卫办函[2013]544号)

摘　　要：目的:探讨葛花总黄酮对大鼠心肌缺血再灌注损伤的保护作用及其作用机制研究。方法:取Wistar大鼠72只,随机分为假手术组,模型组,阳性药组(复方丹参片,240 mg·kg^(-1)),葛花总黄酮高、中、低剂量组(20,40,60 mg·kg^(-1)),预防给药28 d,通过夹闭冠状动脉30 min后恢复供血再灌注的方法复制大鼠心肌缺血再灌注损伤动物模型。造模完成后,通过2,3,5-氯化三苯基四氮唑(TTC)染色测定各组大鼠心肌梗死面积,通过苏木素-伊红(HE)染色观察心肌组织病理性形态学改变,检测血清中总抗氧化能力(T-AOC),水平腺嘌呤核苷三磷酸(ATP),二磷酸腺苷(ADP),天门冬氨酸氨基转移酶(AST),乳酸脱氢酶(LDH),磷酸肌酸激酶(CPK)水平;酶联免疫吸附测定(ELISA)检测心肌细胞组织匀浆液细胞肿瘤因子-α(TNF-α),白细胞介素-6(IL-6),白细胞介素-1β(IL-1β)含量水平;ELISA检测心肌细胞组织细胞凋亡基因B细胞淋巴瘤/白血病-2(Bcl-2),Bcl-2相关X蛋白(Bax),半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)含量表达。结果:与模型组比较,葛花总黄酮高、中剂量组治疗组大鼠心肌梗死面积显著降低(P<0.05,P<0.01);葛花总黄酮高、中剂量治疗组大鼠心肌组织病变和心肌细胞凋亡状况均好转,其中葛花总黄酮高剂量组治疗组效果最为显著;葛花总黄酮高、中剂量治疗组大鼠血清中AST,CPK,ATP,ADP,LDH水平明显降低,心肌组织中TNF-α,IL-6,IL-1β含量明显降低(P<0.05,P<0.01);葛花总黄酮高、中剂量组上调大鼠心肌组织中Bcl-2含量,降低Bax,Caspase-3含量。结论:葛花总黄酮能够降低心肌梗死面积、改善组织病理学改变,提示葛花总黄酮对心肌缺血再灌注大鼠具有保护作用,其作用机制可能与葛花总黄酮能降低炎症细胞因子浸润,降低心肌组织细胞凋亡有关。Objective ： To investigate the protection effect of total flavonoids of Puerariae Lobatae Flos on myocardial ischemia-reperfusion injury in rats and explore its possible mechanism. Method： The 72 Wistar rats were randomly divided into sham operation group, myocardial ischemia-reperfusion model group, positive drug control group （Danshen tablets, 240 mg·kg^-1） , total flavonoids of Puerariae Lobatae Flos low, medium and high dose groups （20, 40, 60 mg·kg^-1）. The drugs were given for 28 days as preventive administration. The rat models were established by clamping the artery for 30 min. After successful modeling, the areas of myocardial infarction were determined by triphenyhetrazolium chloride （TTC） staining; the morphological changes of myocardial tissues were observed by htoxylin eosin （HE） staining; the levels of total antioxidant capacity （T-AOC）, adenosine triphosphate （ATP）, adenosine diphosphate （ADP） , aspartate aminotransferase （AST）, lactate dehydrogenase （LDH）, and creatine phosphokinase （CPK） were detected; the levels of tumor necrosis factor-a （TNF-a）, interleukin-6 （IL-6） and IL-1β in myocardial tissues were determined by enzyme-linked immunosorbent assay （ELISA） ; the levels of Bax, Bcl-2 and Caspase-3 were detected by ELISA. Result： As compared with model group, myocardial infarct size in the total flavonoids of Puerariae Lobatae Flos high dose and middle dose groups was significantly decreased （ P 〈 0.05, P 〈 0.01 ） ; the histopathological changes and cardiomyocytes apoptosis were improved in total flavonoids of Puerariae Lobatae Flos high dose and middle dose groups, and the effect was most obvious in high dose group; the levels of AST, CPK LDH ADP and ATP in serum, as well as TNF-a, IL-6, IL-1β levels in myocardial tissues were decreased significantly in total flavonoids of Puerariae Lobatae Flos high and middle dose groups （P 〈 0.05, P 〈 0.01 ） ; total flavonoids of Puerariae Lobatae Flos high and middle dose groups

关 键 词：葛花总黄酮 心肌缺血再灌注损伤 心肌梗死 组织细胞 炎症细胞浸润 

分 类 号：R285.5[医药卫生—中药学]