自组装短肽GFS-4作为细胞三维培养及心肌梗死修复支架材料的研究  

Self-assembling peptide GFS-4 nanofiber scaffolds for three-dimensional cell cultures and myocardial infarction repair

作　　者：陈振银[1] 岳媛媛[1] 张慧楠[1] 陈春燕[2] 刘博[3] 李雪琴[4] 文静[1] 吴书祎 罗忠礼[1] 

机构地区：[1]重庆医科大学基础医学院分子医学与肿瘤研究中心,重庆400016 [2]郑州人民医院心血管内科第三病区,郑州450000 [3]上海交通大学医学院附属新华医院心血管内科,上海200092 [4]乐山市妇幼保健院检验科,四川乐山614000

出　　处：《生物医学工程学杂志》2017年第3期388-393,共6页Journal of Biomedical Engineering

基　　金：国家自然科学基金(31540019);重庆市科委自然科学基金(cstc2015jcyj BX0072);重庆医科大学科研培育基金(自然科学类)(201417);重庆医科大学基础医学院苗圃基金(JC201514);四川省重点实验室抗生素及其相关研究实验室基金(ARRLKF14-01)

摘　　要：本研究探索自组装短肽GFS-4在心肌细胞三维培养中的应用效果及其对心肌梗死区域的组织修复作用。通过圆二色谱仪分析短肽GFS-4的二级结构,原子力显微镜检测短肽GFS-4自组装的微观形态。将GFS-4自组装形成的纳米纤维支架作为心肌细胞三维培养材料,观察心肌细胞的生长状况;建立大鼠心肌梗死模型,加入水凝胶GFS-4研究其对心肌梗死修复的效果。结果发现,GFS-4自组装形成的二级结构主要为β折叠;自组装24 h后形成致密的纳米纤维支架;心肌细胞三维培养结果表明心肌细胞在GFS-4水凝胶中生长状况良好;心肌梗死体外修复实验发现,短肽GFS-4水凝胶支架可缓解心肌梗死区域组织坏死。自组装短肽GFS-4作为新的纳米支架材料,可用于细胞三维培养和心肌梗死区域组织修复。The purpose of this study is to investigate the effects of self-assembling peptide GFS-4 on three-dimensional myocardial cell culture and tissue repair of myocardial infarction. The circular dichroism （CD） spectrum was used to detect secondary structure of GFS-4, and atomic force microscope （AFM） was used to analyze the microstructure of self-assembly. The nanofiber scaffolds self-assembled by GFS-4 were used as the three-dimensional culture material to observe the growth effect of cardiomyocytes. The model of myocardial infarction was established and the effect of GFS-4 on myocardial infarction was studied. The results indicated that self-assembling peptide GFS-4 could form mainly β-sheet structure that can form dense nanofiber scaffolds after 24 hours＇ self-assembling. The myocardial cells had a favorable growth status in GFS-4 nanofiber scaffold hydrogel when cells treated in three-dimen-sional cell culture. The experiment of repairing myocardial infarction in vitro proved that peptide GFS-4 hydrogel scaffold could alleviate tissue necrosis in a myocardial infarction area. As a new nanofiber scaffold material, self-assembling peptide GFS-4 can be used for three- dimensional cell culture and tissue repairing in myocardial infarction area.

关 键 词：自组装短肽 GFS-4 细胞三维培养 心肌梗死修复 

分 类 号：R318.08[医药卫生—生物医学工程] R542.22[医药卫生—基础医学]