青海地区藏族人群IFN-γ、TNF-α及IL-10基因多态性与乙型肝炎病毒感染易感性的关系  

Association between the susceptibility of HBV infection and gene polymorphisms of IFN-γ,TNF-α and IL-10 in the Tibetan population in Qinghai area

作　　者：任利[1,2] 路越晴 程冠[1] 于文昊 王芝 王聪[1,2] 鄂伟健 王海久[1,2] 周瀛[1,2] 阳丹才让 侯立朝 樊海宁[1,2] 

机构地区：[1]青海大学附属医院肝胆胰外科,青海西宁810001 [2]青海省包虫病研究重点实验室 [3]青海省第四人民医院 [4]青海大学附属医院检验科

出　　处：《实用预防医学》2017年第7期814-818,共5页Practical Preventive Medicine

基　　金：国家自然科学基金资助项目(81060133)

摘　　要：目的探讨乙型病毒性肝炎易感性与IFN-γ、TNF-α及IL-10基因单核苷酸多态性的相关性,旨在基因层面确立青海地区藏族人群HBV易感性的影响因素。方法收集来青海大学附属医院和青海省第四人民医院就诊的藏族人群HBV感染患者血样258例作为病例组,从体检中心收集的健康人群血样126例作为对照组。DNA提取试剂盒提取外周血基因组DNA,用飞行质谱技术检测IFN-γ(+874A/T、+2109C/T)、TNF-α(-308)、IL-10(-592,-1082)多态性位点的碱基及基因型,并分析与乙型肝炎病毒性易感性的相关性。结果 TNF-α(-308)和IL-10(-592,-1082)多态性位点的碱基及基因型频率分布在病例组(92.4%、7.6%;24%、52%、24%,1.2%、23.2%、75.6%)与对照组(92.5%、7.5%;23.9%、46.3%、29.8%,4.5%、19.4%、76.1%)分布差异无统计学意义(P>0.05)。尽管IFN-γ多态性位点+2109C/T的基因型和碱基及IFN-γ+874A/T位点的基因型在对照组(14.3%、43.7%、42%;2.4%、19.8%、77.8%)和病例组(10.5%、47.3%、42.2%;3.1%、30.6%、66.3%)的频率分布差异无统计学意义(P>0.05),但IFN-γ+874A/T位点的A碱基在病例组的频率(18.4%)分布显著高于对照组(12.3%)(P<0.05),T碱基在对照组的频率(87.7%)分布显著高于病例组(81.6%),差异有统计学意义(P<0.05)。结论在青海藏族人群,IFN-γ+874A/T位点的等位基因T可能是HBV感染的保护性因素,而等位基因A是HBV感染的易感因素。Objective To explore the genetic correlations between the susceptibility to hepatitis B virus （ HBV ） and single nu- cleotide polymorphisms of IFN-γ, TNF-α and IL-10 so as to identify the influencing factors of HBV susceptibility at the genetic level in Tibetan population in Qinghai area. Methods We collected 258 blood samples from Tibetan patients infected with HBV in the Affiliated Hospital of Qinghai University and the Fourth People＇ s Hospital of Qinghai Province to serve as the case group. And 126 blood samples from healthy individuals in the Physical Examination Center were collected as the control group. Genomic DNA was extracted from the peripheral blood by DNA extraction kit. The bases and genotypes of gene polymorphism loci of IFN-γ（＋874A/T, ＋2 109C/T）, TNF-α （-308） and IL-10 （-592, -1,082） were detected by flight mass spectrometry, and their correlations with the susceptibility to hepatitis B virus were analyzed. Results No statistically significant differences were found in the frequency distribution of bases and genotypes of gene polymorphism loci of TNF-α （-308） and IL-10 （ -592, -1 082） be- tween the case group （ 92.4%, 7.6% ; 24%, 52%, 24% ; 1.2%, 23.2%, 75.6% ） and the control group （ 92.5%, 7.5% ; 23.9%, 46.3%, 29.8%; 4.5%, 19.4%, 76.1%; all P〉0.05）. Although the frequency distribution of bases and genotypes of IFN-γ ＋ 2109C/T and IFN-γ ＋874A/T polymorphism loci in the control group （ 14.3%, 43.7%, 42%; 2.4%, 19.8%, 77.8%） and the case group （ 10.5%, 47.3%, 42.2% ; 3.1%, 30.6%, 66.3%） showed no statistically significant differences （ all P〉0.05） , but the frequency distribution of base A of IFN- γ＋874A/T loci was significantly higher in the case group than in the control group （18.4% vs. 12.3%） and base T was significantly higher in the control group than in the ease group （87.7% vs. 81.6%） （both P 〈0.05）. Conclusions For the Tibetan population in Qinghai Province, the allele T of IFN-γ＋874A/T may be the protect

关 键 词：干扰素-Γ 肿瘤坏死因子-α 白介素-10 乙肝 基因多态性 

分 类 号：R181.24[医药卫生—流行病学]