不同剂量氟化钠对Balb／c小鼠软骨损害及白细胞介素-6表达的影响  

Effects of different doses of sodium fluoride on cartilage lesion and expression of interleukin-6 in Balb/c mice

作　　者：杨庆[1] 褚衍茹 江伟[1] 李俊骏 李悦[1] 包莹[1] 陈富讯[2] 李冰洋[2] 杨艳梅[2] 高彦辉[1] 

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心地氟病防治研究所、环境相关疾病研究所,哈尔滨150081 [2]哈尔滨医科大学中国疾病预防控制中心地方病控制中心中心实验室,哈尔滨150081

出　　处：《中华地方病学杂志》2017年第6期408-413,共6页Chinese Journal of Endemiology

基　　金：黑龙江省杰出青年基金（JC2015018）

摘　　要：目的 探讨不同剂量氟化钠对Balb／c小鼠软骨损害以及对小鼠血清、软骨组织中白细胞介素-6（interleukin-6，IL-6）表达的影响。方法 64只5周龄雄性Balb／c小鼠，按体重（18 ~ 21 g）采用随机数字表法分成4组，每组16只。对照组饮用蒸馏水，低、中、高氟组分别饮用含氟量为25、50、100 mg／L的蒸馏水。每周称量1次小鼠体重，饲养3个月建立饮水型氟中毒模型。处死小鼠，氟离子选择电极法检测脊柱骨氟含量；光镜下观察膝关节软骨、骺板软骨的病理变化；酶联免疫吸附法检测血清IL-6和可溶性IL-6受体（sIL-6R）含量；免疫组化法检测膝关节软骨、骺板软骨IL-6蛋白表达情况。结果 与其他3组比较，高氟组小鼠自染氟第6周，体重开始下降（P均 〈 0.05）；与对照组和低氟组比较，中氟组小鼠自染氟第7周，体重开始下降（P均 〈 0.05）；与对照组比较，低氟组小鼠在整个染氟过程中体重变化不明显（P均 〉 0.05）。对照组和低、中、高氟组小鼠骨氟含量［（842.46 ± 89.27）、（1 705.05 ± 105.76）、（2 614.17 ± 156.10）、（3 444.58 ± 233.69）mg／kg］组间比较差异有统计学意义（F = 309.716，P 〈 0.05），且随着染氟剂量的增加而增加（P均 〈 0.05）。光镜下，对照组软骨组织结构正常；染氟组关节软骨、骺板软骨呈现不同程度的软骨内骨化，且随着染氟剂量的增加而加重。对照组和低、中、高氟组小鼠血清IL-6水平［（5.98 ± 1.43）、（7.54 ± 2.16）、 （5.25 ± 1.97）、（6.31 ± 1.36）ng／L］组间比较差异有统计学意义（F = 3.840，P 〈 0.05），其中低氟组明显高于对照组（P 〈 0.05），中氟组明显低于低氟组（P 〈 0.05）。对照组和低、中、高氟组小鼠血清sIL-6R水平［（0.83 ± 0.20）、（0.93 ± 0.23）、（0.82 ± 0.27）、（0.92 ± 0.28）μg／L］组间比较差异无统计学意义（F = 0.738，P �Objective To explore the effects of different doses of sodium fluoride （NaF） on cartilage lesion and expression of interleukin-6 （IL-6） in serum and cartilage tissue of Balb/c mice. Methods Sixty-four 5-week-old male Balb/c mice were divided into 4 groups based on body weight via the random number table method and 16 mice were in each group. The mice in control group were fed with distilled water, and experimental animals in low, middle and high fluoride groups were fed with distilled water containing NaF 25, 50 and 100 mg/L, respectively. The mice were weighed once a week and fed for three months to establish the drinking water fluorosis model. The fluoride contents in spine were detected via the fluorin-ion selective electrode method. The pathological changes in articular cartilage and epiphyseal plate cartilage were observed through optical microscope. The levels of serum IL-6 and souble IL-6 receptor （sIL-6R） were detected via the enzyme-linked immunosorbent assay. The expression of IL-6 protein in articular cartilage and epiphyseal plate cartilage was examined by immunohistochemistry. Results From the sixth week of the experiment, compared with other 3 groups, the body weight of high fluoride group decreased significantly （all P 〈 0.05）; from the seventh week, compared with control and low fluoride groups, the body weight of middle fluoride group decreased significantly （all P 〈 0.05）; throughout the experiment, compared with control group, the body weight of low fluoride group had not changed significantly （all P 〉 0.05）. The fluoride contents of bone in control group, low fluoride group, middle fluoride group and high fluoride group were （842.46 ± 89.27）, （1 705.05 ± 105.76）, （2 614.17 ± 156.10） and （3 444.58 ± 233.69） mg/kg, respectively. The differences between groups were statistically significant （F = 309.716, P 〈 0.05）, and fluoride contents of bone increased with increase of fluoride doses （all P 〈 0.05）. Under optical microscope, the cart

关 键 词：氟化钠 软骨 白细胞介素-6 小鼠 

分 类 号：R599.1[医药卫生—内科学]