定量计算机断层扫描测量氟中毒家兔骨密度及与骨损伤相关性分析  

Quantitative computed tomography measurement skeletal fluorosis rabbits bone density and the correlation with bone injury

作　　者：蒋菲菲[1] 雷平贵 陈义家[1] 邹迅[2] 罗鹏[1] 潘雪莉[1] 

机构地区：[1]贵州医科大学公共卫生学院,环境污染与疾病监控教育部重点实验室,贵阳550025 [2]贵州医科大学附属医院医学影像科,贵阳550004

出　　处：《中华地方病学杂志》2017年第6期414-417,共4页Chinese Journal of Endemiology

摘　　要：目的 分析定量计算机断层扫描（QCT）骨密度改变与氟中毒家兔骨损伤的相关性。方法 取家兔16只，雌雄各半，按体重采用随机数字表法分为对照组和实验组，每组8只，分别饮用含氟化钠0、300 mg／L的自来水（0、663 g分析纯氟化钠溶于1 000 ml自来水）。90 d后，检测家兔活体股骨骨密度（QCT测定）和骨损伤的相关指标［氟离子选择电极法测定家兔股骨骨氟含量，酶联免疫吸附法（ELISA）测定家兔血清碱性磷酸酶（ALP）及骨钙素（BGP），光镜下观察股骨病理学改变并计算骨小梁面积］。结果用独立样本校正t（t＇）检验和偏相关性检验进行统计学分析。结果 实验组骨密度［（653.49 ± 167.81）g／cm3］、骨氟含量［（3 232.16 ± 927.85）mg／kg］，血清ALP［（42.69 ± 3.28）U／L］、BGP［（2 504.19 ± 276.79）μg／L］，骨小梁面积［（39.02 ± 3.33）Tb.Ar］均高于对照组［（540.40 ± 41.99）g／cm3、（554.01 ± 376.51）mg／kg、（20.50 ± 4.90）U／L、（1 294.60 ± 191.86）μg／L、（8.15 ± 2.34）Tb.Ar，t＇ = 2.615、7.565、10.641、10.158、14.494，P均 〈 0.05］。实验组股骨QCT影像可见成像关节面组织硬化，骨质硬化、骨纹理粗化。光镜下实验组股骨表现为骨小梁数目增多，排列紊乱。相关性分析发现，骨密度与骨氟、骨小梁面积、BGP、ALP均呈正相关［相关系数（r） = 0.702、0.627、0.614、0.567，P均 〈 0.05］。结论 QCT可测量氟中毒家兔三维骨质密度，其与骨氟含量、骨组织病理学改变、骨代谢指标均具有良好相关性，可为QCT在氟骨症患者中的应用提供有效参考。Objective To detect the early diversification of the bone mineral density in skeletal fluorosis of rabbits by quantitative computed tomography （QCT）, and analyze the possible relationship between bone mineral density and bone injury in rabbits with fluorosis. Methods A total of 16 rabbits, half male and half female, were randomly divided into control and experimental groups according to body weight. The two groups of rabbits were given drinking water containing NaF 0 or 300 mg/L, respectively, for 90 days. After the experiment, their bone fluoride content was determined via the fluorine ion-selective electrode method. The alkaline phosphatase （ALP） and osteocalcin （BGP） in serum were measured using enzyme-linked immunosorbent assay （ELISA）. Femurl bone mineral density was detected with QCT in vivo. Histopathological changes of femur were observed under light microscope and trabecular acreage was calculated. The results were analyzed with independent-samples t test（t＇） and partial correlations. Results The bone fluoride content [（3 232.16 ± 927.85） mg/kg], ALP [（42.69 ± 3.28） U/L], BGP concentration [（2 504.19 ± 276.79） μg/L], bone density [（653.49 ± 167.81） g/cm3] and trabecular number [（39.02 ± 3.33）Tb.Ar] of the experimental group were higher than those of control group [（554.01 ± 376.51）mg/kg, （20.50 ± 4.90）U/L, （1 294.60 ± 191.86）μg/L, （540.40 ± 41.99）g/cm3, （8.15 ± 2.34）Tb.Ar], and the differences were statistically significant （t＇ = 7.565, 10.641, 10.158, 2.615, 14.494, all P 〈 0.05）. The tissue sclerosis, bone sclerosis and bone texture coarsening were observed through bone mineral density imaging taken by QCT in experimental group. The number of trabeculae increased and the arrangement of trabeculae was disorganized. Bone mineral density was positively correlated with bone fluoride, trabeculae, BGP and ALP （r = 0.702, 0.627, 0.614, 0.567, all P 〈 0.05）. Conclusions QCT bone density measurement in skeletal fluorosis of

关 键 词：氟化物中毒 定量计算机断层扫描 骨密度 创伤和损伤 

分 类 号：R599.1[医药卫生—内科学]