大花益母草的化学成分研究  

Chemical constituents from aerial parts of Leonurus macranthus

作　　者：黄正[1,2] 霍会霞 任易[1,2] 彭治添 晁凌会 孙慧[1,2] 刘文静[1,2] 张云封[1,2] 赵云芳[1] 屠鹏飞[1] 李军 

机构地区：[1]北京中医药大学中药现代研究中心,北京100029 [2]北京中医药大学中药学院,北京100102

出　　处：《中草药》2017年第9期1724-1729,共6页Chinese Traditional and Herbal Drugs

基　　金：教育部新世纪优秀人才支持计划资助项目(NCET-13-0693);国家"重大新药创制"科技重大专项资助项目(2013ZX09402201001)

摘　　要：目的研究大花益母草Leonurus macranthus的化学成分。方法采用硅胶、Sephadex LH-20、ODS柱色谱和半制备HPLC等色谱技术进行分离纯化,并根据理化性质和波谱数据鉴定化合物的结构。结果从大花益母草地上部分70%丙酮提取物的二氯甲烷萃取部位分离鉴定了19个化合物,分别鉴定为(+)-丁香脂素(1)、(+)-1-羟基丁香脂素(2)、rayalinol(3)、erythro-guaiacylglycerol-β-O-4′-coniferyl ether(4)、(7R,7′R,7″S,8S,8′S,8″S)-3′,4″-dihydroxy-3,5,4′,5″-tetramethoxy-7,9′:7′,9-diepoxy-4,8″-oxy-8,8′-sesquineo-lignan-7″,9″-diol(5)、(7R,7′R,7″S,8S,8′S,8″S)-4′,5″-dihydroxy-3,5,3′,4″-tetramethoxy-7,9′:7′,9-diepoxy-4,8″-oxy-8,8′-sesquineo-lignan-7″,9″-diol(6)、芫花素(7)、3′-羟基芫花素(8)、圣草酚(9)、异莨菪亭(10)、对香豆酸(11)、咖啡酸甲酯(12)、反式-阿魏酸(13)、丁香醛(14)、香草酸(15)、oct-1-en-3-ylβ-glucopyranoside(16)、5-羟基-2-吡咯烷酮(17)、pterolactam(18)和烟酰胺(19)。结论化合物1~6和9~19为首次从益母草属植物中分离得到,化合物7和8为首次从大花益母草中分离得到。Objective To investigate the chemical constituents from the aerial parts of Leonurus macranthus. Methods The chemical constituents were separated and purified by silica gel, Sephadex LH-20, ODS column chromatographies, and semi-preparative HPLC. Their structures were determined by physicochemical properties and spectroscopic data. Results Nineteen compounds were isolated from the CH2Cl2 layer of 70% aqueous acetone extract in the aerial parts of L. macranthus, and identified as（＋）-syringaresinol（1）,（＋）-1-hydroxysyringaresinol（2）, rayalinol（3）, erythro-guaiacylglycerol-β-O-4′-coniferyl ether（4）,（7R,7′R,7″S,8S,8′S,8″S）-3′,4″-dihydroxy-3,5,4′,5″-tetramethoxy-7,9′：7′,9-diepoxy-4,8″-oxy-8,8′-sesquineo-lignan-7″,9″-diol（5）,（7R,7′R,7″S,8S,8′S,8″S）-4′,5″-dihydroxy-3,5,3′,4″-tetramethoxy-7,9′：7′,9-diepoxy-4,8″-oxy-8,8′-sesquineo-lignan-7″,9″-diol（6）, genkwanin（7）, 3′-hydroxygenkwanin（8）, eriodictyol（9）, isoscopoletin（10）, p-coumaric acid（11）, caffeic acid methyl ester（12）, trans-ferulic acid（13）, syringic aldehyde（14）, vanillic acid（15）, oct-1-en-3-yl β-glucopyranoside（16）, 5-hydroxy-2-pyrrolidone（17）, pterolactam（18）, and nicotinamide（19）, respectively. Conclusion Compounds 1—6 and 9—19 are isolated from the plants of genus Leonurus Linn. for the first time, and compounds 7 and 8 are found fromL. macranthus for the first time.

关 键 词：大花益母草 (+)-丁香脂素 圣草酚 异莨菪亭 对香豆酸 丁香醛 香草酸 芫花素 3′-羟基芫花素 

分 类 号：R284.1[医药卫生—中药学]