宝儿康散大鼠ig给药后血浆暴露成分分析  

Analysis on components exposing in rat plasma after oral administration of Baoerkang Powder

作　　者：何坤明[1,2] 刘延平[2] 杨元辉[2] 魏广力[2] 李全胜[2] 陈勇[1] 司端运[2] 

机构地区：[1]湖北大学中药生物技术省重点实验室,湖北大学生物资源绿色转化协同创新中心,湖北武汉430062 [2]天津药物研究院释药技术与药代动力学国家重点实验室,天津300193

出　　处：《中草药》2017年第9期1740-1747,共8页Chinese Traditional and Herbal Drugs

摘　　要：目的分析大鼠ig宝儿康散后在血浆中的暴露成分,为其作用机制及药动学研究奠定基础。方法采用超高效液相色谱-高分辨飞行时间质谱联用技术(UPLC-Q-TOF/MS),分离给药后吸收入血成分。根据化合物的保留时间,相对分子质量及二级质谱特征碎片,结合文献报道,分析鉴定大鼠血浆中宝儿康散化学成分。结果大鼠ig宝儿康散1 h后,在血浆中分离鉴定了23个化合物,其中有5个化合物通过与对照品比对保留时间与质谱数据,确定了化学结构,分别为牡荆素、甘草素、橙皮素、甘草次酸、熊果酸。结论本方法可以快速分析宝儿康散大鼠ig给药后血浆暴露成分,对进一步探究其作用机制及其药动学奠定了基础。Objective To analyze the components exposing in rat plasma after oral administration of Baoerkang Powder, and to study the mechanism of Baoerkang Powder and pharmacokinetic behavior. Methods Components absorbed in rat plasma after oral administration of Baoerkang Powder to rats were analyzed by UPLC tandem High-resolution mass spectrometer. The structures of Baoerkang Powder in rat plasma identified by comparing the retention time, molecular weight, and CID fragmentation patterns with their corresponding compounds reported in the literatures. Results Twenty-three components in rat plasma were identified after oral administration of Baoerkang Powder to rats for 1 h, including five components confirmed by comparing retention time and information of mass with their reference substances, components confirmed were vitexin, liquiritigenin, hesperetin, glycyrrhetic acid, and ursolic acid. Conclusion It is suggested that the method could be applied to quick analysis of the components exposing in rat plasma after oral administration of Baoerkang Powder, which is beneficial to studying its mechanism and pharmacokinetic behavior.

关 键 词：宝儿康散 高分辨质谱 血浆暴露成分 牡荆素 甘草素 橙皮素 甘草次酸 熊果酸 

分 类 号：R284[医药卫生—中药学]