朱砂与万氏牛黄清心丸致大鼠亚急性肝毒性的比较研究  

Comparative study of subacute liver toxicity in rats induced by cinnabar and Wanshi Niuhuang Qingxin Pills

作　　者：黄海 高鑫 周颖 高显会 王莹 

机构地区：[1]锦州医科大学,辽宁锦州121001

出　　处：《中草药》2017年第9期1825-1828,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81503209);辽宁省教育厅一般项目(L2015320)

摘　　要：目的比较朱砂和万氏牛黄清心丸(含朱砂的成方制剂)对大鼠的亚急性肝毒性。方法分别以溶剂(0.5%羧甲基纤维素钠水溶液)、1.0 g/(kg·d)朱砂、9.83 g/(kg·d)万氏牛黄清心丸[全方,含朱砂1.0 g/kg]、8.83 g/(kg·d)去朱砂的万氏牛黄清心丸(去朱砂方)对健康SD大鼠ig给药,每天1次,连续28 d。给药结束后次日取血液和肝脏,检测血汞、肝汞水平,检测血清肝功能指标,进行肝组织病理学观察。结果朱砂组血汞、肝汞和血清总蛋白(TP)水平高于对照(溶剂)组、全方组和去朱砂方组(P<0.05);光镜下,朱砂组可见轻度的肝细胞肿胀、空泡变性、炎症细胞浸润等病理改变,全方组和去朱砂方组可见色素沉积,病变轻微,偶见肝细胞肿胀、空泡变性。结论短期过量使用朱砂可引起肝汞蓄积和轻微肝损伤,万氏牛黄清心丸引起的肝汞蓄积量和亚急性肝损伤轻于单味朱砂,方中其他中药可能对朱砂起到减毒作用。Objective To compare the subacute liver toxicity of cinnabar and Wanshi Niuhuang Qingxin Pills, one of Chinese patent medicines containing cinnabar, in rats. Methods Healthy SD rats were given by gavage solvent（0.5% sodium carboxymethyl cellulose）, 1.0 g/（kg·d） cinnabar, 9.83 g/（kg·d） Wanshi Niuhuang Qingxin Pills [the whole recipe containing 1.0 g/（kg·d） cinnabar], 8.83 g/（kg·d） Wanshi Niuhuang Qingxin Pills without cinnabar recipe, once daily for 28 d. The rats were sacrificed the next day after administration, and blood and liver samples were collected for the detection of mercury content, indexes of liver function and liver histopathology. Results The contents of liver mercury, blood mercury, and serum total protein in cinnabar group were higher than those of the control group, the whole recipe group, without cinnabar recipe group（P〈0.05）. Under the light microscope, mild liver pathological changes were observed in the cinnabar group, including slight hepatocyte swelling and vacuolization, occasionally infiltration of inflammatory cells. Pigmentation and slight pathological changes, such as hepatocyte swelling and vacuolization, were observed in the whole recipe group and without cinnabar recipe group. Conclusion Short-term overdose of cinnabar could cause mercury accumulation in liver and result in slight liver damage. The accumulation of mercury and subacute injury in liver induced by Wanshi Niuhuang Qingxin Pills is slighter than by single cinnabar.

关 键 词：朱砂 万氏牛黄清心丸 肝毒性 亚急性 汞蓄积 

分 类 号：R285.5[医药卫生—中药学]