马鞭草HPLC-CAD指纹图谱及主要成分的测定  

Chromatographic fingerprint and multi-components quantitative analysis of Verbena officinalis by HPLC-CAD

作　　者：陈学松 

机构地区：[1]广西壮族自治区梧州食品药品检验所,广西梧州543002

出　　处：《中草药》2017年第10期2007-2011,共5页Chinese Traditional and Herbal Drugs

摘　　要：目的建立马鞭草Verbena officinalis的指纹图谱及同时测定马鞭草中同分异构体齐墩果酸和熊果酸的方法。方法分析柱为Thermo Fisher Acclaim_(TM) C_(30)(150 mm×2.1 mm,3μm)色谱柱;流动相为乙腈-0.2%醋酸水溶液,梯度洗脱;体积流量为0.3 m L/min;进样量为5μL;柱温20℃;电雾式检测器检测,雾化器温度为40℃。结果 5批不同产地的马鞭草指纹图谱有17个共有峰,共有峰相对保留时间RSD值小于0.35%,相对峰面积RSD值在3.3%～4.40%。5批马鞭草药材中齐墩果酸的量在0.097%～0.136%,熊果酸的量在0.257%～0.478%,总量在0.354%～0.478%。结论首次用高效液相色谱-电雾式检测(HPLC-CAD)方法建立了马鞭草的指纹图谱并同时测定了马鞭草药材中的齐墩果酸和熊果酸的量。该方法简便快捷、重复性好,可用于马鞭草的质量控制。Objective To establish a high performance liquid chromatography-charged aerosol detector （HPLC-CAD） method in order to obtain fingerprint profile and achieve the amount of isomer, oleanolic acid, and ursolic acid from Verbena officinalis. Methods The method was developed by a C30 （150 mm × 2.1 mm, 3 μm） column and a linear gradient elution. Acenitrile-0.2% acetic acid was used as mobile phase. The flow rate was 0.3 mL/min; The injection volume was 10μL and the column temperature was maintained at 20℃. Detector was Corona Ultra CAD with 40℃ of nebulization temperature. Results Seventeen common peaks were observed from the fingerprint profiles of five different area samples. The relative standard divisions （RSDs） of retention time were less than 0.35%. The relative peak areas of the common peaks were in the range of 3.3%--44.0%. The contents of oleanolic acid were ranging from 0.097% to 0.136%, the contents of ursolic acid were ranging from 0.257% to 0.478%, the total contents were ranging from 0.354% to 0.478%. Conclusion The fingerprint and simultaneous determination of oleanolic acid and ursolic acid in V. ojficinalis is firstly studied by HPLC-CAD. This method is simple and with good repeatability, which can be used for the quality control of E officinalis.

关 键 词：马鞭草 指纹图谱 高效液相色谱-电雾式检测器 齐墩果酸 熊果酸 

分 类 号：R286.6[医药卫生—中药学]