农产品中白藜芦醇的高效液相色谱标准化测定方法研究  

Determination of Resveratrol in Agricultural Products Using HPLC Standardized Method

作　　者：潘葳[1] 刘文静[1] 涂杰峰[1] 

机构地区：[1]福建省农业科学院农业质量标准与检测技术研究所/福建省精密仪器农业测试重点实验室,福州350003

出　　处：《中国农学通报》2017年第14期130-137,共8页Chinese Agricultural Science Bulletin

基　　金：福建省地方标准制订项目"项目名称"(闽质监标[2011]595号)

摘　　要：旨在建立同时测定花生衣、葡萄皮、苜蓿、虎杖、藜芦等农产品中白藜芦醇含量的高效液相色谱标准化测定方法。色谱分析条件如下:以Waters Atlantis T3-C18色谱柱(4.6×150mm,3μm)分离,柱温40℃;以乙腈:0.1%乙酸溶液(体积比为26:74)为流动相,流速1.0 mL/min;采用二极管阵列检测器,检测波长306 nm。花生衣、葡萄皮、苜蓿中白藜芦醇含量的检出限均为0.6μg/g,定量限均为2.0μg/g;虎杖、藜芦中白藜芦醇含量的检出限均为7.5μg/g,定量限均为25μg/g。方法学考察结果显示符合含量测定要求。该方法操作简便、快速,结果可靠,重现性好,可用来研究农产品中白藜芦醇的含量。A high performance liquid chromatographic （HPLC） standardized method was established to simultaneous determine the content of resveratrol in agricuhural products such as peanut skin, grape skin, alfalfa, Polygonum cuspidatum and Veratrum nigrum Linn.. The HPLC conditions were as follows： Waters Atlantis T3 C18 column （4.6 mm i.d.× 150 mm, 3 μm） was used at 40℃ with aeetonitrile-water containing 0.1% acetic acid （26：74, v/v） as the mobile phase with a flow rate of 1.0 mL/min, diode array detector with the detection at 306 nm. The limits of detection （LODs） and limits of quantification （LOQs） for peanut skin, grape skin and alfalfa were 0.6 μg/g and 2.0 μg/g. LODs and LOQs for Polygonum euspidatum and Veratrum nigrum Linn. were 7.5μg/g and 25 μg/g. The results of methodological study demonstrated that the method met the requirements of the determination. The method was simple, fast, accurate and reproducible for the analysis of resveratrol in agrieuhural products.

关 键 词：高效液相色谱法 白藜芦醇 农产品 

分 类 号：O657[理学—分析化学]