鸡传染性法氏囊病活疫苗(B87株+CA株+CF株)安全性及免疫效果评价  

Safety and Immune Effect Evaluation of Infectious Bursal Diesease Live Vaccine(Strain B87+Strain CA+Strain CF)

作　　者：李润 孙晔[2] 赵洪明 梁占军 王荣申 蒋桃珍[2] 

机构地区：[1]北京中海生物科技有限公司,北京100081 [2]中国兽医药品监察所,北京100081 [3]石家庄市动物疫病预防控制中心,石家庄050000 [4]无极县畜牧兽医站,石家庄052460

出　　处：《中国兽药杂志》2017年第6期1-8,共8页Chinese Journal of Veterinary Drug

基　　金：石家庄市科学技术研究与发展计划(131200562A)

摘　　要：通过观察疫苗免疫后鸡群的生长情况和临床症状,检测免疫后不同时期鸡群的ELISA抗体水平和攻毒保护率以及对鸡新城疫(ND)疫苗免疫后不同时间鸡群的HI抗体水平,评价和检测了鸡传染性法氏囊病活疫苗(B87株+CA株+CF株)在石家庄地区临床应用的安全性、免疫效果、免疫持续期以及是否会产生免疫抑制。结果表明,试验疫苗接种蛋鸡和肉鸡后,均没有观察到因疫苗引起的不良反应,对蛋鸡和肉鸡均安全。疫苗接种蛋鸡后14 d、28 d、42 d、60 d和90 d,接种肉鸡后14 d、21 d和36 d攻毒保护率均达80%以上;商品蛋鸡接种疫苗后90 d其ELISA抗体水平仍高达6500以上。ND HI抗体检测结果表明,试验疫苗接种后对ND疫苗的免疫应答无显著影响,不引起免疫抑制。试验疫苗安全、有效,质量稳定,适用于预防鸡传染性法氏囊病。By observing the growth and clinical symptoms of chickens after vaccine immunization, the ELISA antibody level, immune protection rate, and the HI antibody level of chickens after injection of ND vaccine in different periods were detected to evaluate the safety, immune effect, immune duration, and immunosuppression of clinical application of the IBD live vaccine （Strain B87＋Strain CA＋Strain CF） in Shijiazhuang area. The results showed that the experimental vaccines were safe to layer and broiler chickens as no adverse reaction from the vaccine injection had been observed. The rate of challenging protection were all above 80% for laying hens on day 14, 28, 42, 60 and 90 after immunization and the broiler chickens on day 14, 21 and 36 after immunization as well. The ELISA antibody level was still more than 6500 on the 90 days after immunization of commercial laying hens. According to the detection results of ND HI antibody, injection of the experiment vaccines made no significant effect on the response of the immunity of ND vaccine and did not cause any immunosuppression. The experimental vaccines were adapt to prevention of IBD for their safety, efficiency and stable quality.

关 键 词：鸡传染性法氏囊病 疫苗 免疫效果 

分 类 号：S858.315.3[农业科学—临床兽医学]