江西柿属种质资源遗传多样性的IRAP标记分析  

Genetic Diversity Analysis of Diospyros spp. Germplasm Resources in Jiangxi by Using IRAP Molecular Marker

作　　者：曾明[1] 杜晓云[1] 吴美华[1] 王彦波[1] 

机构地区：[1]江西省农业科学院园艺研究所,江西南昌330200

出　　处：《江西农业学报》2017年第6期29-34,共6页Acta Agriculturae Jiangxi

基　　金：江西省自然科学基金项目(20151BAB204031);江西省杰出青年科学家资助计划项目(20162BCB23054);江西省科技支撑计划重点项目(20151BBF60009);江西省农科院创新基金项目(2013CQN003);江西省农科院科技创新及成果转化基金项目(优势创新团队)(2015CJJ001);江西现代农业科研协同创新专项(JXXTCX2015006-003)

摘　　要：利用逆转座子间扩增多态性(Inter-retrotransposon Amplified Polymorphism,IRAP)分子标记对收集的49份江西柿属种质资源进行了遗传多样性分析。从26个IRAP引物中筛选出扩增条带清晰且多态性丰富的10个引物,PCR扩增产物经聚丙烯酰胺凝胶电泳(PAGE)得到清晰的指纹图谱。将418条清晰条带(多态性100%)转换为0/1矩阵,聚类分析结果表明:供试的江西柿属种质资源的遗传相似系数在0.69~0.92之间,分属柿、油柿、野柿三个种,遗传多样性丰富;明晰了几个分类关系模糊的柿种质资源的种属关系。The genetic diversities of 49 germplasm resources in Diospyros in Jiangxi were analyzed by using IRAP （ Inter-ret- rotransposon Amplified Polymorphism） molecular marker. Ten primers which had clear bands and polymorphism in PCR amplifi- cation were screened out from 26 IRAP primers, and the PCR-amplified products presented clear fingerprint by polyacrylamide gel electrophoresis （PAGE）. The 418 clear bands （100% diversity） were transformed into 0/1 matrix, and the clustering analysis showed that the genetic similarity coefficients among the tested 49 germplasm resources varied from 0.69 to 0.92, and these germplasm resources belonged to 3 species （ Diospyros kaki Thunb., Diospyros oleifera Cheng, and Diospyros kaki var. silves- tis Makino）, which revealed abundant genetic diversity. In addition, the species taxonomic relationships among several Diospyros spp. germplasm resources were clarified.

关 键 词：江西 柿属 种质资源 遗传多样性 IRAP 

分 类 号：S665.2[农业科学—果树学]