青枯病植物疫苗对番茄根系土壤微生物群落结构的影响  

Effects of Avirulent Ralstonia solanacearum Strain as Plant Vaccine on Microbial Community in Rhizosphere Soil of Tomato

作　　者：郑雪芳[1] 刘波[1] 朱育菁[1] 

机构地区：[1]福建省农业科学院农业生物资源研究所,福州350003

出　　处：《中国生物防治学报》2017年第3期385-393,共9页Chinese Journal of Biological Control

基　　金：国家公益性行业(农业)科研专项(201303015);福建省自然科学基金(2015J01103);福建省农业科学院青年人才创新基金(YC2016-15)

摘　　要：为了探讨青枯雷尔氏菌无致病力菌株FJAT-1458作为青枯病植物疫苗菌株对番茄根系土壤微生物群接种处理后番茄根系土壤微生物PLFAs组成、含量、微生物群落结构及多样性的变化。结果表明,与清水对照相比,接种菌株FJAT-1458后,番茄根系土壤微生物脂肪酸组成及含量发生了明显变化,可分为减少型、无变化型和增加型。进一步分析表明,菌株FJAT-1458能促进番茄根系土壤微生物总量、细菌总量和真菌总量的增加,增加幅度分别为5.98%~47.36%、2.07%~58.07%和5.65%~74.42%,对放线菌生长起到先抑制后促进作用。此外,接种菌株FJAT-1458后番茄根系土壤微生物群落的Shannon-wiener指数显著增加,21 d增加量最高(13.32%)。聚类分析表明,菌株FJAT-1458处理组与对照组的番茄根系土壤微生物均划分为3个群落类型(兰氏距离分别为3.28和4.62),但它们各自群落组成及特征不同;同时主成分分析也将2种处理划分在2个不同类群中。上述结果说明菌株FJAT-1458处理能明显改变番茄根系土壤微生物群落结构,增加其多样性,从而提高土壤微生态系统的稳定性,增强土壤抑制病害能力。In order to explore the effect of avirulent Ralstonia solanacearum strain FJAT-1458 as plant vaccine on microbial community structures in tomato rhizosphere soil, tomato plants were inoculated by FJAT-1458 with water as a control. Then the rhizosphere soil was sampled at 3, 6, 9, 12, 15, 18 and 21 d after treatment, respectively. Phospholipid fatty acids （PLFAs） of soil samples were detected using Agilent 6890 N. The results showed that PLFAs composition and content in tomato rhizosphere soil were significantly altered by inoculation of FJAT-1458, which could be divided into decreasing type, unchanging type and increasing type. Further statistical analysis showed that the inoculation of FJAT-1458 could increase the content of total microbes, bacteria and fungi by 5.98%-47.36%, 2.07%-58.07% and 5.65%-74.42%, respectively. Growth of actinomycetes was inhibited at the beginning and then improved by FJAT-1458 inoculation. Moreover, inoculation of FJAT-1458 could increase the microbial community diversity in tomato rhizosphere soil, and the enhancement of Shannon-wiene index was the most significant, with the highest value of 13.32% at 21 d after inoculation. Cluster analysis revealed that the microbe in tomato rhizosphere soil both in FJAT-1458 treatment and control could be divided into three communities at 3.28 and 4.62 Lance distance, but constitutes and characters of PLFAs between the two treatments were different. Principal component analysis demonstrated that the two treatments could also be distinguished into two different groups. All these suggested that inoculation of FJAT-1458 could alter microbial community structure in tomato rhizosphere soil and increase its diversity, and further improve the stability of soil micro-ecosystem and the ability for bacterial wilt disease control.

关 键 词：植物疫苗 番茄 土壤微生物群落结构 磷脂脂肪酸 

分 类 号：S476[农业科学—农业昆虫与害虫防治]