Optimization of IBRS-2 Cells Culturing Conditions for Cultivation of Porcine Parvovirus N Strain Using Roller Bottle  

应用正交试验优化猪细小病毒N株在IBRS-2细胞转瓶培养(英文)

作　　者：卢冰霞 何颖 赵武 梁家幸 段群棚 蒋冬福 卢敬专 闭炳芬 周英宁 秦毅斌 李斌 苏乾莲 陈忠伟 

机构地区：[1]广西壮族自治区兽医研究所、广西兽医生物技术重点实验室,广西南宁530001

出　　处：《Agricultural Science & Technology》2017年第5期839-842,共4页农业科学与技术（英文版）

基　　金：Supported by Nonprofit Institute Research Grant of Guangxi Province(GK15-2,GK16-2);Guangxi Aquatic Animal Husbandry Science and Technology Project(GYMK201528030,201633034)~~

摘　　要：The aim was to optimize the roller bottle culture system of IBRS-2 cells for cultivation of porcine parvovirus （PPV） N strain using a four-factor three-level or- thogonal test （cell culture medium pH value： 7.0, 7.2 and 7.4; inoculation time： 0, 24 and 48 h; inoculation dose： 0.10%, 1.00% and 10.00%; harvest time： 48, 72 and 96 h）. The results showed that the optimal conditions were as follows： cell culture medium pH value of 7.2, synchronous inoculation, inoculation dose of 0.10% and culture time of 96 h. Among the four factors, culture time was the most important factor affectina the virulence titer of PPV N strain.利用正交试验对猪细小病毒(PPV)N株在IBRS-2细胞转瓶培养中的培养条件进行优化,为PPV N株弱毒疫苗的大量培养工艺提供参考。设细胞培养液p H值(7.0、7.2和7.4)、接毒时间(0、24和48 h)、接毒剂量(0.10%、1.00%和10.00%)、收毒时间(48、72和96 h)等因子和水平。结果表明,PPV N株转瓶培养的最佳培养条件组合为细胞培养液p H值7.2,采用同步接毒,接毒剂量为0.10%,收毒时间为接毒后96 h。其中收毒时间是影响PPV N株毒价的最主要因素。

关 键 词：Porcine parvovirus （PPV） Roller bottle culture OPTIMIZATION Orthogonal test 

分 类 号：S852.651[农业科学—基础兽医学]