百脉根4个共生基因在水稻中的共表达及其对水稻转录组的影响  

Coexpression of four symbiotic genes fromLotus japonicus in rice and its effects on rice transcriptome

作　　者：李文清 曹文刚 李豪[1] 方庆[1] 张忠明[1] 

机构地区：[1]农业微生物学国家重点实验室/华中农业大学生命科学技术学院,武汉430070

出　　处：《华中农业大学学报》2017年第4期55-61,共7页Journal of Huazhong Agricultural University

基　　金：国家重点基础研究发展计划“973”项目(2010C126502);高等学校博士学科点专项(20130146130003)

摘　　要：为建立水稻-根瘤菌共生固氮体系,将百脉根共生信号转导途径中的4个功能基因(LHK1、NSP1、NSP2和NIN)转入水稻,获得稳定转化的转基因水稻,并初步检测转基因水稻的表型和转录组水平上的差异。根瘤菌感染试验结果显示,根瘤菌侵染对照(转入空载体)和转基因水稻的根毛和表皮的比率没有显著变化,但接种根瘤菌14d后,对照与转基因水稻的侧根原基数有显著差异,转共生基因水稻的侧根原基明显增多。利用水稻cDNA芯片,检测转基因水稻组与对照组在接种根瘤菌7d和不接种根瘤菌条件下根的转录组变化,结果显示,转基因组与对照组水稻在植物防御反应、激素信号转导途径相关基因均有差异性表达,表明豆科共生基因影响了水稻对根瘤菌的反应。To explore the possibility of establishing the rice-rhizobium symbiotic nitrogen fixation system, four genes （LHK 1, NSP 1, NSP 2 and NIN） of symbiosis signaling in Lotus japonicus were cotransformed into rice. Transgenic rice plants were obtained. After inoculation with rhizobium,there was no significant difference in the root hairs and epidermis infected by rhizobia of the control and the trans- genic plants. The number of lateral root and primordia in transgenic plants was significantly increased compared with those in the control after inoculation 14 days. The results of transcriptional analysis using rice cDNA microarray showed that expression of those genes involved in plant defense response and phytohormone signaling was significantly up-regulated in the transgenic plants. It is indicated that leguminous symbiotic genes affect rice response to rhizobia.

关 键 词：百脉根 根瘤共生 转基因水稻 根瘤菌 

分 类 号：Q939.9[生物学—微生物学]