葛根素对糖尿病大鼠心肌保护作用的机制研究  

PROTECTIVE EFFECT OF PUERARIN ON MYOCARDIUM OF DIABETIC RATS

作　　者：高静媛 杨雨旸 潘彩飞 孙尧 李继安 王志文 

机构地区：[1]华北理工大学附属医院老年病科,河北省唐山市063000 [2]华北理工大学中医学院

出　　处：《中国煤炭工业医学杂志》2017年第6期707-709,共3页Chinese Journal of Coal Industry Medicine

摘　　要：目的探讨葛根素对糖尿病大鼠心肌的保护作用,并探讨其作用机制。方法一次性腹腔注射链脲佐菌素(STZ)诱导雄性SD大鼠。取造模成功的大鼠40只,随机分成四组:模型组、葛根素低剂量组(40mg/kg)、葛根素中剂量组(80mg/kg)、葛根素高剂量组(160mg/kg),每组10只。另取同龄正常饲养大鼠10只作为对照组。按组别给予不同药物干预12周后分别测定大鼠血清Ang-Ⅱ水平,测定舒张末期室间隔厚度(IVSTd)、舒张末期左心室后壁厚度(LVPWTd)、左室舒张末期内径(LVEDd)、收缩末期左心室内径(LVEDs)及左室射血分数(LVEF),HE染色观察心肌组织病理学改变。结果与模型组比较,葛根素各组大鼠Ang-Ⅱ水平下降,以葛根素高剂量组下降最明显(P<0.05);与模型组相比,葛根素各组IVSTd、LVPWTd、LVEDd及LVEDs降低,其中以葛根素高剂量组变化更为明显(P<0.05);葛根素高剂量组心肌组织结构紊乱程度减轻,纤维组织增生减轻,中性粒细胞聚集减少。结论葛根素可抑制肾素血管紧张素系统,并通过此机制对心脏起保护作用。Objective To evaluate the protective effect of Puerarin on myocardium of diabetic rats and its mechanism.Methods Forty rats were intraperitoneally injected with streptozocin （STZ） to induce rats. Forty diabetic rats were randomly divided into four groups： model group,low dose group of Puerarin （40mg/kg） , middle dose group （80mg/kg） ,high dose group （160mg/kg）. Ten normal control rats were used as control group.serum Ang- Ⅱ levels were determined after 16 weeks of different drug intervention. The level of IVSTd, LVPWTd, LVEDd, LVEDs and LVEF was determined, and the histopathological changes of myocardium by HE staining were observed. Results Compared with the model group,the level of Ang - Ⅱ was decreased in Puerarin treatment group, and the high dose group of Puerarin decreased most obviously （P 〈0.05） ;Compared with the model group, IVSTd, LVPWTd, LVEDd and LVEDs decreased in Puerarin treatment group,and the high dose group of Puerarin decreased most obviously （P 〈0.05）. Puerarin high dose group reduced the degree of disorder of myocardial tissue structure, reduced the proliferation of fibrous tissue, and reduced the accumulation of neutrophils.Conclusion Puerarin can inhibit renin angiotensin system and protect the heart through this mechanism.

关 键 词：糖尿病 葛根素 Ang-Ⅱ 

分 类 号：R587.1[医药卫生—内分泌]