电生不稳定试剂化学发光法测定盐酸异丙嗪  

Chemiluminescence Determination of Promethazine Hydrochloride With Electrogenerated Unstable Reagent

作　　者：黄嘉初[1] 

机构地区：[1]安徽六安师范专科学校

出　　处：《广西民族学院学报（自然科学版）》1998年第2期30-31,35,共3页Journal of Guangxi University For Nationalities(Natural Science Edition)

基　　金：国家自然科学基金

摘　　要：首次将恒电流电解产生ＣｌＯ－，与流动注射化学发光结合，提出电生不稳定试剂ＣｌＯ－化学发光分析法，很好地解决了化学发光反应中次氯酸盐不稳定问题，并对具有还原性药物进行测定，方法基于还原性药物能抑制ＣｌＯ－—ｌｕｍｉｎｏｌ电化学发光，其浓度与抑制作用的大小呈浅性关系．利用该法测定盐酸异丙嗪，发现盐酸异丙嗪在４×１０－９～６×１０－８ｇ／ｍｌ浓度范围内与空白、试样信号差值呈线性关系，检出限为７．６×１０－１０ｇ／ｍｌ，相对标准偏差为２．９％．It is first time that a electro generated unstable reagent chemiluminescence system used in determination of promethazine hydrochloride with reversed flow injection analysis. The method of constant current electrolysis generated Clo - solved a problem on hypochlorite unstable. The system was used in determination of some medicines. It is based on the inhibition of some medicines in electrochemiluminescence reaction of Clo --luminol system. The system has been applied to the assay of promethazine hydrochloride in injection. The linear calibration range is 4×10 -9 ～6×10 -8 g/mL and the detection limit is 7 6×10 -10 g/mL. The relative standard deviation is 2 9%.

关 键 词：电生不稳定试剂 化学发光法 盐酸异丙嗪 含量测定 抗组织胺药物 次氯酸根离子 

分 类 号：R976[医药卫生—药品] R927.2[医药卫生—药学]