p38MAPK特异性抑制剂SB203580对糖尿病小鼠肾小球滤过率的影响  

作　　者：赵咏莉[1] 姚新明[1] 陈月平[1] 何春玲[1] 翟清[1] 王勇[1] 

机构地区：[1]皖南医学院弋矶山医院,241000

出　　处：《浙江临床医学》2017年第7期1191-1193,共3页Zhejiang Clinical Medical Journal

基　　金：安徽省自然科学基金资助项目（1608085QH191）;安徽省芜湖市皖南医学院校中青年科研基金（WK200938F）

摘　　要：目的探讨p38丝裂原活化蛋白激酶（p38MAPK）特异性抑制剂SB203580对糖尿病小鼠肾小球滤过率的影响及机制。方法60只清洁级小鼠随机分为正常对照组（NG组）、糖尿病组（DM组）和SB203580治疗组（SB组）。测定小鼠血清超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量、尿白蛋白排泄和肾小球滤过率。结果与NG组比较，DM组血清SOD活性明显降低，MDA含量和尿白蛋白排泄明显增加（P〈0．05）；与DM组比较，SB组血清SOD活性升高，MDA含量和尿白蛋白排泄降低（P〈0．05）。与NG组比较，DM组肾小球滤过率降低（P〈0．05）；与DM组比较，SB组肾小球滤过率升高（P〈0．05）。相关性分析：肾小球滤过率与血清SOD活性存在正相关关系（r=0．670，P〈0．001），与血清MDA含量存在负相关关系（r=-0．731，P〈0．001）。结论p38MAPK特异性抑制剂SB203580通过减轻氧化应激改善肾小球滤过率。Objective To observe the effect of p38 mitogen-activated protein kinase （ p38MAPK ） specific inhibitor SB203580 on glomerular filtration rate in diabetic mice. Methods Sixty clean mice were randomly divided into normal control group （ NG group ） , diabetic group （ DM group ） and SB203580 treatment group （ SB group ） . The activity of superoxide dismutase （ SOD ） , content of malondialdehyde （ MDA ） , urinary albumin excretion and glomerular filtration rate were measured. Results （ 1 ） Compared with NG group, the activity of SOD in DM group was significantly decreased, the content of MDA and urinary albumin excretion were significantly increased （ P〈0.05 ） . Compared with DM group, the activity of SOD in SB group was increased, the content of MDA and urinary albumin excretion were decreased （ P〈0.05 ） . （ 2 ） The glomerular filtration rate of DM group was lower than that of NG group （ P〈0.05 ） , and the glomerular filtration rate of SB group was higher than that of DM group （ P〈0.05 ） . （ 3 ） Correlation analysis： The glomerular filtration rate was positively correlated with the activity of SOD （ r=0.67, P〈0.001 ） , and the glomerular filtration rate was negatively correlated with the content of MDA （ r=-0.731, P〈0.001 ） . Conclusion p38MAPK specific inhibitor SB203580 improves glomerular filtration rate by reducing oxidative stress.

关 键 词：P38MAPK 氧化应激 糖尿病小鼠 肾小球滤过率 

分 类 号：R971[医药卫生—药品]