实验性NOD小鼠慢性淋巴细胞性甲状腺炎造模建立及其机制研究  

作　　者：叶志鹏[1] 吴可人[1] 方蓉[1] 李宁[1] 徐涛[1] 金法[1] 

机构地区：[1]浙江中医药大学附属第一医院,310006

出　　处：《浙江临床医学》2017年第7期1200-1202,共3页Zhejiang Clinical Medical Journal

基　　金：浙江省中医药科技计划项目（2A11414）

摘　　要：目的探讨NOD小鼠慢性淋巴细胞性甲状腺炎（CLT）建模方法及其机制研究。方法NOD小鼠20R，随机分为2组，对照组予每天饮用蒸馏水，而造模组饮用0．05％碘水（0．64gNaI／L），持续8N后取甲状腺组织及血浆，观察两组小鼠甲状腺体组织病理改变和血浆抗小鼠TG抗体水平变化；ELlSA测定小鼠脾脏中IL-4或IFN-1，分析碘剂对CLT小鼠Th1／Th2细胞比值的影响。结果（1）与对照组比较，造模组炎性细胞浸润程度较大，且差异有统计学意义（P〈0．05）。（2）血浆中血浆抗小鼠TG抗体OD值，造模阻比对照组有明显升高（P〈0．05）。（3）与对照组比较，造模组中IL-4明显下降（P〈0．05），IFN-γ明显升高（P〈0．01），IFN-γ／IL-4比值升高（P〈0．05）。结论碘剂诱导小鼠慢性淋巴细胞性甲状腺炎造模建立是可行的。过量碘剂能通过促进IFN-γ等Th1细胞因子分泌，抑制IL-4等Th2细胞因子分泌，使Th1／Th2细胞平衡向Th1方向偏离，形成免疫性甲状腺炎。Objective To investigate mechanism research and modeling method of Hashimoto＇ s Thyroiditis of experimental NOD mice. Method 20 NOD mice were divided equally into model group and normal group, and were separately fed with diet of iodine （ 0.64g NaI/L ） and distilled water.Eight weeks later, thyroid tissue and plasma were collected to observe the histopathological changes of thyroid gland and the level of anti TG antibody in plasma. IFN-γ and interleukin-4 （ IL-4 ） produced by splenocytes were measured by enzyme-linked immunosorbent assay. Result （ 1 ） Compared with control group by histopathology detection, NOD mice developed CLT in normal group was less than that of the model group, which was obviously different （ P〈0.05 ） . （ 2 ） There was a difference in serum mTgAb among the both groups, and had statistical significance. （P〈0.05） . （ 3 ） In the model groups, the IL-4 was lower, IFN-γ and IFN-γ/IL-4 were higher than that of the normal group. Conclusion It is feasible to establish a model of chronic lymphocytic thyroiditis induced by iodine in mice. Adequate iodine can promote the secretion ofThl cytokine including IFN- γ and suppress Th2 cytokine including IL-4, which results in the disorder of Th1/Th2, and even Thl cells take a more important role.

关 键 词：桥本甲状腺炎 碘剂 造模 TH细胞 

分 类 号：R581.4[医药卫生—内分泌]