柱前衍生HPLC法测定五谷虫中脂肪酸含量  

Determination of fatty acid content in the maggots by pre column derivation HPLC

作　　者：刘敏[1] 刘静[2] 刘新光[2] 李斌[2] 刁云鹏[2] 李坤[1] 

机构地区：[1]辽宁师范大学化学化工学院,辽宁大连116029 [2]大连医科大学药学院,辽宁大连116044

出　　处：《吉林医药学院学报》2017年第3期169-171,共3页Journal of Jilin Medical University

摘　　要：目的建立HPLC法测定五谷虫中脂肪酸含量的方法。方法采用2-溴代苯乙酮作为衍生化试剂,三乙醇胺作为催化剂对脂肪酸进行衍生化使其产生紫外吸收,利用HPLC法进行含量测定。色谱条件为Agilent-C18柱(250 mm×4.6 mm,5μm);检测波长254 nm;流动相为水∶甲醇=10∶90;柱温为20℃;体积流量为1 m L/min,进样量为20μL,内标为十七烷酸。结果在上述色谱条件下,各脂肪酸衍生物均达到基线分离。亚油酸的含量为131.0 mg/L,软脂酸的含量为194.8 mg/L,油酸的含量为100.6 mg/L,硬脂酸含量为36.6 mg/L。结论该方法稳定,重现性好,可用于五谷虫脂肪酸含量测定。Objective To develop a HPLC method for the determination of fatty acid extracted from moggots. Methods Fatty acids were derivatized with 2-Bromoacetophenone and triethano]amine as the eatalyst to produce UV absorption, and the content was determined by HPLC method. Chromatographic conditions were as following：chromatogram column ： Agilent-C18 column （250 mm×4.6 mm,5μm） ; detection wavelength ： 254 nm ; mobile phase ： water： methanol = 10：90 ; column temperature ： 20℃ ; flow rate ： 1 mL/min, sample volume ： 20 μL; internal standard ： seventeen alkyl acid. Results Under the above chromatographic conditions, all the fatty acid derivatives reached the baseline separation. The content of linoleic acid was 131.0 mg/L, and the palmitic acid was 194.8 mg/L,the oleic acid was 100.6 mg/L,while the stearic acid was 36.6 mg/L. Conclusion This method is stable and reproducible, and could be used for the determination of fatty acid extracted from maggots.

关 键 词：五谷虫 脂肪酸 柱前衍生 高效液相 

分 类 号：R286.02[医药卫生—中药学]