白芍总苷治疗大鼠急性痛风性关节炎的作用及对血清IL-1β、IL-6、TNF-α的影响  

Effect of total glucosides of peony therapy on acute gouty arthritis and serum levels of IL-1β,I L-6 and TNF-α in rat models

作　　者：李振彬[1] 马旭[1] 杨静[1] 刘彦卿[1] 路占忠 李达[1] 刘乐[1] 刘芝[1] Li Zhenbin Ma Xu Yang Jing Liu Yanqing Lu Zhamhong Li Da Liu Le Liu Zhi.(Department of Rheumatology and Immunology, Bethune International Peace Hospital of PLA, Shijiazhuang 050082, Chin)

机构地区：[1]中国人民解放军白求恩国际和平医院风湿免疫科,石家庄050082

出　　处：《新医学》2017年第6期380-384,共5页Journal of New Medicine

基　　金：河北省科技计划项目(13277704D);河北省中医药管理局科研计划课题(2015180)

摘　　要：目的观察白芍总苷对大鼠急性痛风性关节炎(AGA)的防治效果及对AGA大鼠血清IL-6、IL-1β、TNF-α水平的影响。方法选取雄性SD大鼠48只,随机分为对照组、模型组、秋水仙碱0.8 mg/kg组、白芍总苷低、中、高剂量(75、150、300 mg/kg)组,共6组,每组各8只大鼠。给药5 d后,采用踝关节注射微晶型尿酸钠(MSU)混悬液法建立AGA大鼠模型。检测基线期及造模后不同时间点的踝关节直径,以造模后与基线期踝关节直径的差值作为关节肿胀度。试验结束后,大鼠腹主动脉采血,ELISA检测血清IL-6、IL-1β、TNF-α的含量,比较组间差异。结果与对照组相比,模型组大鼠造模后不同时间点的关节肿胀度均增加(P均<0.01);与模型组相比,白芍总苷低、中、高剂量组及秋水仙碱组在造模后各时间点的关节肿胀度均降低(P均<0.05),各治疗组间比较差异无统计学意义(P>0.05)。与对照组相比,模型组大鼠血清IL-6、IL-1β、TNF-α水平升高(P均<0.01);造模后24 h,白芍总苷低、中、高剂量组与秋水仙碱组大鼠血清IL-6、IL-1β、TNF-α水平较模型组降低(P均<0.01),而各治疗组间比较差异均无统计学意义(P均>0.05)。结论白芍总苷对AGA大鼠有治疗作用,其作用机制可能与抑制MSU诱导的促炎细胞因子IL-6、IL-1β、TNF-α的产生有关。Objective To evaluate the effect of total glucosides of peony upon acute gouty arthritis （AGA） and serum levels of IL-6, IL-1β and TNF-α in AGA rat models. Methods Forty-eight male SD rats were chosen and randomly divided into the control, model, colchicine （0. 8 mg/kg）,low, moderate and high dose （75,150 and 300 mg/kg） of total glucosides of peony groups （n = 8 for each group）. At 5 d after medi-cine administration, AGA rat models were established by injection of microcrystalline monosodium urate （MSU） suspension in the ankle joint. The diameter of ankle joint at baseline and different time points after model establishment was measured. The difference in the ankle joint diameter before and after model establish-ment was used to reflect the degree of knee joint swelling. After experiment, blood sampling was collected via the abdominal aorta. The serum levels of IL-6, IL-1β and TNF-α were quantitatively measured by using ELISA and statistically compared among different groups. Results Compared with the control group, the degree of knee joint swelling in the model group was significantly enhanced at different time points （all P 〈0. 01）. Compared with the model group, the degree of joint swelling in the low-, moderate- and high-dose total glucosides of peony, and colchicine groups was significantly decreased at different time points （all P 〈0. 05）. No statistical significance was identified among different treatment groups （all P 〉0. 05）. Compared with the control group, the serum levels of IL-6, IL-1 p and TNF-a in the model group were significantly up-regulated （ all P 〈 0. 01）. At 24 h after model establishment, the serum levels of IL-6, IL-1β and TNF-α in the low-, moderate- and high-dose total glucosides of peony and colchicine groups were considerably down-regulated than those in the model group （ all P 〈 0. 01）. No statistical significance was documented among different treatment groups （ all P 〉 0. 05）. Conclusion Use of

关 键 词：白芍总苷 急性痛风性关节炎 肿瘤坏死因子-Α 白介素-1Β 白介素-6 大鼠 

分 类 号：R285.5[医药卫生—中药学]