不同白毛夏枯草提取物抗肝癌机制分析  

Analysis of mechanism of anti-hepatoma different ajugadecumbens extractive

作　　者：吴航[1] 令小峰 Wu Hang Ling Xiaofeng(Qinghat Hospital of TCM（Xining 810000)

机构地区：[1]青海省中医院,西宁810000 [2]西宁市食品药品监督管理局,西宁810008

出　　处：《陕西中医》2017年第6期812-814,共3页Shaanxi Journal of Traditional Chinese Medicine

摘　　要：目的:以白毛夏枯草水提取物、乙酸乙酯提取物为例探究不同提取物的抗肝癌机制。方法:将白毛夏枯草的水提取物(STW)、乙酸乙酯提取物(ZTW)分别作用于肝癌细胞株HepG2和SMMC-7721。运用四甲基偶氮唑盐(MTT)法检测不同浓度的STW、ZTW对肝癌细胞株HepG2和SMMC-7721增生的抑制作用,通过流式细胞仪检测不同浓度的STW、ZTW诱导肝癌HepG2和SMMC-7721细胞凋亡的凋亡率,运用一步法荧光RT-PCR(Real-time PCR)技术检测经不同浓度的STW、ZTW处理后HepG2和SMMC-7721细胞中Bax mRNA的表达情况。结果:STW、ZTW对HepG2和SMMC-7721细胞均有显著的抑制增生和诱导其凋亡的作用,同时能够抑制肝癌血管增殖。STW、ZTW的抑制机制呈一定的浓度依赖性,其中以乙酸乙酯提取物ZTW抑制性最强。结论:白毛夏枯草水提取物、乙酸乙酯提取物均通过抑制肝癌细胞增生,诱导细胞凋亡,抑制肝癌血管增殖,加速肝癌细胞分化等机制来抗肝癌。ObjectiveTo mechanism of anti-hepatoma ajugadecumbens water extractive and ethyl acetate ex-tract as an example to explore the different extracts. Methods：The water extract of Herba etate extract （ZTW） respectively in HepG2 and SMMC-7721 in hepatocellular carcinoma cell yl thiazolyl tetrazolium （MTT） method was used to detect the inhibitory effect of different concentration of STW,ZTW and SMMC-7721 on HepG2 cell proliferation, apoptosis and apoptosis of hepatoma SMMC-7721 cells induced by HepG2 under different concentrations of STW and ZTW were detected by flow cytometry, using one-step fluores-cent RT-PCR （Real-time PCR） expression technique for the detection of the different concentrations of STW, ZTW and HepG2 after treatment of SMMC-7721 cells inBax mRNA. Results ： STW andZTW could signiticathe proliteration and induce apoptosis of HepG2 and SMMC-7721 cells, and inhibit the proliteration of hepatoma cells. The inhibitory mechanism of STW and ZTW was concentration dependent, and was the strongest. Conclusion： Herba Ajugae water extract and ethyl acetate extract by inhibiting proliferation ofhepatoma cells, inducecell apoptosis, inhibit vascular proliferation, accelerate cell differentiation mechanisms a-gainst liver cancer.

关 键 词：白毛夏枯草 提取物 抗肝癌机制 

分 类 号：R282[医药卫生—中药学]