饲料中添加合成虾青素对中华绒螯蟹成体雌蟹性腺发育、色泽和抗氧化能力的影响  

EFFECTS OF DIETARY SUPPLEMENTATION OF SYNTHETIC ASTAXANTHIN ON OVARIAN DEVELOPMENT, COLORATION AND ANTIOXIDANT CAPACITY OF ADULT FEMALE CHINESE MITTEN CRAB,ERIOCHEIR SINENSIS

作　　者：麻楠[1] 龙晓文[1] 赵磊[1] 常国亮[3] 吴旭干[1,2] 成永旭[1,2] MA Nan LONG Xiao-Wen ZHAO Lei CHANG Guo-Liang WU Xu-Gan CHENG Yong-Xu(Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai201306, China Collaborative Innovation Center of Aquatic Animal Breeding Center Certificated by Shanghai MunicipalEducation Commission, Shanghai Ocean University, Shanghai 201306, China Jiangsu Engineering Laboratory forCharacteristic Aquatic Species Breeding, Huaiyin Normal University, Huai’an 223300, China)

机构地区：[1]上海海洋大学农业部淡水水产种质资源重点实验室,上海201306 [2]上海海洋大学,上海市教委水产动物遗传育种协同创新中心,上海201306 [3]淮阴师范学院,江苏省特色水产繁育工程实验室,淮安223300

出　　处：《水生生物学报》2017年第4期755-765,共11页Acta Hydrobiologica Sinica

基　　金：科技部港澳台科技合作专项项目(2014DFT30270);上海市科学技术委员会科研项(13320502100,16DZ2281200);上海高校水产学一流学科建设项目(2012-62-0908);深圳澳华农牧科技有限公司委托项目(D-8006-15-0054);江苏省科技厅政策引导类产学研合作项目(BY2016062)资助~~

摘　　要：采用在基础饲料中分别添加0、130和260 mg/kg的合成虾青素配制成3种粗蛋白和粗脂肪含量分别为42%和16%的等氮等脂的中华绒螯蟹(Eriocheir sinensis)育肥饲料(分别记为饲料1、2和3),以中华绒螯蟹商业育肥饲料作为饲料4,分别投喂4组雌蟹(每个饲料组3个重复水槽,每个水槽中12只蟹),进行为期60d的室内养殖实验,以探讨添加合成虾青素对中华绒螯蟹成体雌蟹性腺发育、色泽及抗氧化能力的影响。结果显示:(1)实验进行到30d和60d,在饲料中添加虾青素对雌体肝胰腺指数(HSI)和性腺指数(GSI)均无显著影响(P>0.05)。(2)性腺、肝胰腺和头胸甲中的总类胡萝卜素含量和红度值(a值)均以饲料3组最高,性腺亮度值(L值)和黄度值(b值)以饲料1组最高(P<0.05);饲料2组头胸甲的b值最高,饲料1组最低(P<0.05)。(3)饲料1组中华绒螯蟹血清过氧化物酶(POD)活性显著高于其他组,饲料4组的过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)、乳酸脱氢酶(LDH)、总抗氧化能力(T-AOC)、丙二醛(MDA)和乳酸(LD)含量最高(P<0.05);肝胰腺中的SOD、T-AOC、GSH-Px和GR活性均以饲料1组最高,饲料2组最低(P<0.05)。(4)饲料2组血清酸性磷酸酶(ACP)活性和血蓝蛋白(Hc)含量显著高于其他组,其余各组间差异不显著。血清中的碱性磷酸酶(ALP)和γ-谷氨酰转移酶(γ-GT)活性和一氧化氮(NO)含量均以饲料4组最高(P<0.05),肝胰腺中的ACP、ALP和γ-GT活性以饲料1组最高。综上,在育肥饲料中添加合成虾青素对成体雌蟹性腺发育无显著影响,但可显著提高头胸甲、肝胰腺和卵巢中的类胡萝卜素总量、色泽和抗氧化能力,建议雌蟹育肥饲料中合成虾青素的含量为90 mg/kg左右。To investigate the effects of dietary supplementation of synthetic astaxanthin on ovarian development, colora-tion and antioxidant capacity of adult female Chinese mitten crab （Eriocheir sinensis）, three isonitrogenous and isoli-pidic fattening diets with 42% crude protein and 16% crude lipid were formulated by adding 0, 130 and 260 mg/kg syn-thetic astaxanthin （named as Diet 1, Diet 2, and Diet 3, respectively）, and commercial fattening diet was used （named asDiet 4）. Each diet was fed to triplicate tanks with 12 female crabs per tank for 60-day fattening. The results showed thatdietary supplementation of synthetic astaxanthin had no significant effect on the hepatosomatic index （HSI） and gon-adosomatic index （GSI） of adult female E. sinensis （P〉0.05）. Diet 3 treatment had the highest contents of carotenoidand redness value （a）, and Diet 1 group had the highest lightness value （L） and yellow value （b） in the ovaries. Thehighest and the lowest b value of carapace were found in Diet 2 and Diet 1 treatments, respectively （P〈0.05）. Diet 1group had the highest activity of peroxidase （POD） in the serum. The activities of catalase （CAT）, glutathione re-ductase （GR）, lactic dehydrogenase （LDH）, total antioxidant capacity （T-AOC） and the contents of malondialdehyde（MDA） and lactic acid （LD） in serum from Diet 4 group were significantly higher than those of other groups （P〈0.05）.The highest and lowest activities of superoxide dismutase （SOD）, T-AOC, glutathione peroxidase （GSH-Px） and GR inhepatopancreas were found in Diet 1 treatment and Diet 2 treatment, respectively （P〈0.05）. The activity of acid phos-phatase （ACP） and hemocyanin （Hc） content in the serum from Diet 2 treatment were signifi-cantly higher than those ofother treatments （P〈0.05）. The highest activities of alkaline phosphatase （ALP） and γ-glutamyl transpeptidase （γ-GT）,and nitric oxide （NO） content were found in Diet 4 treatment. The highe

关 键 词：合成虾青素 中华绒螯蟹 雌体 性腺发育 色泽 抗氧化能力 

分 类 号：S968.25[农业科学—水产养殖]