白芍水溶性成分快速鉴定研究  

Analysis and Identification of Water-Soluble Components of Paeonia lactiflora

作　　者：谭淑瑜[1] 肖雪[1] 朴胜华[1] 李莎莎[2] 

机构地区：[1]广东药科大学中医药研究院,广东广州510006 [2]广东省中医院,广东广州510006

出　　处：《中药材》2017年第1期94-100,共7页Journal of Chinese Medicinal Materials

摘　　要：目的:利用超高效液相色谱-飞行时间质谱(UPLC-Q-TOF-MS)对白芍水提物中的化学成分进行分析和鉴定。方法:色谱条件:色谱柱:Waters ACQUITY^(TM) BEH C_(18)(100 mm×2.1 mm,1.7μm),流动相为0.1%甲酸水溶液(A)-甲醇(B),梯度洗脱,流速0.4 m L/min,柱温30℃,进样量5μL。质谱条件:AB Sciex Triple TOF 5600质谱仪,ESI离子源,正、负离子模式扫描。结果:通过分析正、负离子质谱信息及元素组成,并结合相关文献,共鉴定出69个化学成分。结论:通过UPLC-Q-TOF-MS技术,为鉴定白芍建立了一种快速、高效的分析方法。Objective ：To analyze and identify the chemical compositions from water extract of Paeonia lactiflora by ultra perform- ance liquid chromatography-time of flight mass spectrometry（UPLC-Q-TOF-MS）. Methods：An ACQUITYTM BEH C18 column（ 100 mm ×2. 1 mm） with 1.7 μm particle size was used. The mobile phase was composed of methanol and water with 0. 1% formic acid in gradi- ent mode. The flow rate was 0.4 mL/min, the column temperature was 30℃ and the injection volume was 5 μL. An AB Sciex Triple TOF 5600 and ESI ion source was used for mass spectra. The MS data were collected in both positive and negative modes. Results：The results showed that 69 compounds from water extract of Paeonia lactiflora had been identified by analyzing mass data and comparing with literatures. Conclusion ： A simple and reliable method with the usage of UPLC-Q-TOF-MS is established to identify the water-soluble components of Paeonia lactiflora.

关 键 词：白芍 超高效液相色谱-飞行时间质谱 成分鉴定 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]