天芪益智颗粒对鹅膏蕈氨酸致痴呆大鼠模型抗氧化能力的影响  

Effects of Tianqiyizhi granules on antioxidant ability in AD rats induced by Ibotenic acid

作　　者：郑航[1,2] 魏江平[1,2] 陈欢[1,2] 付文君[1,2] 张占军[3] 徐世军[1,2] 

机构地区：[1]四川省中药资源系统研究与开发利用国家重点实验室培育基地,成都611137 [2]成都中医药大学药学院,成都610075 [3]北京师范大学认知神经科学与学习国家重点实验室,北京100875

出　　处：《中药药理与临床》2017年第1期155-157,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家科技重大专项-重大新药创制(2013ZX09103002-002);四川省学术和技术带头人培养资金资助项(2015058);中药药理四川省青年科技创新研究团队(2014TD0007)

摘　　要：目的:探讨天芪益智颗粒对痴呆模型大鼠认知功能及抗氧化能力的影响。方法:建立鹅膏蕈氨酸基底核微量注射AD模型。采用Morris水迷宫法检测大鼠的学习记忆能力变化;尼氏染色法检测海马和大脑皮层神经元病理改变;分光度法检测大鼠脑组织MDA、H_2O_2含量及SOD、GR、CAT活力。结果:与模型组相比,天芪益智颗粒0.63g/kg、0.42g/kg剂量组第5d逃避潜伏期明显降低,0.42g/kg剂量组穿越平台次数明显增加,神经元数量增多;与模型组比较,天芪益智颗粒0.63g/kg、0.42g/kg剂量组显著降低MAD、H_2O_2含量及提高GR活力。结论:天芪益智颗粒具有改善模型大鼠认知功能障碍,保护神经细胞的作用,该作用与其增强抗氧化能力有关。Objective： To investigate effects of Tianqiyizhi Granules on cognitive function and antioxidant ability in AD rats.Methods： SD rats were micro-injected with Ibotenic acid into their basal ganglia to establish AD models.Learning and memory ability were tested by the Morris water maze test; neurons in the hippocampus and cerebral cortex were analyzed by nissl staining; The levels of MAD,H2O2 and activities of SOD,GR,CAT in rat brain tissue were detected with spectrophotometry.Results： Compared with the model group,Tianqiyizhi Granules at 0.63g/kg,0.42g/kg dose could significantly reduce the escape latency on 5th day,Tianqiyizhi Granules at 0.42g/kg dose could significantly increase the crossing platform frequency and the number of neurons rose.Compared with the model group,Tianqiyizhi Granules at 0.63g/kg,0.42g/kg dose could significantly reduce the levels of MAD,H2O2 and significantly improve the activity of GR,and also could slightly increase the activity of SOD,CAT.Concusion： Tianqiyizhi Granules could ameliorate cognitive impairment and protect neurons through enhancing antioxidant ability.

关 键 词：天芪益智颗粒 阿尔茨海默病 鹅膏蕈氨酸 

分 类 号：R285.5[医药卫生—中药学]