大黄-黄芪不同剂量配比对慢性肾功能衰竭大鼠肠粘膜和肠道菌群移位的影响  

Effects of different ratios of rhubarb and astragale on intestinal mucosa and bacterial translocation in chronic renal failure rats

作　　者：钟瑜萍 郑作亮 李海燕[1] 宫仁豪 涂祎珺 赖有勤 张煜民 

机构地区：[1]广州中医药大学,广州510006

出　　处：《中药药理与临床》2017年第2期130-133,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：2014年国家自然科学基金青年基金项目(81403122)

摘　　要：目的:探讨大黄-黄芪不同剂量配比对慢性肾衰竭大鼠肠粘膜和肠道菌群移位的影响。方法:选取已建立慢性肾衰模型的大鼠分为模型组、贝那普利(5 mg/kg)组、大黄(1.5 g/kg)组、大黄-黄芪1:1(3.0 g/kg)组、大黄-黄芪1:2(4.5 g/kg)组、大黄-黄芪1:4(7.5 g/kg)组、大黄-黄芪1:6(10.5 g/kg)组。用显色基质鲎试剂终点显色法、生物素双抗体夹心酶联免疫吸附法、蛋白免疫印迹法和细菌平板计数法分别检测血浆内毒素、血清D-乳酸、回肠组织中occludin蛋白相对表达量及肠道菌群移位量。结果:(1)慢性肾衰模型组大鼠较假手术组大鼠内毒素、肠道细菌移位率显著升高,occludin蛋白相对表达量显著降低,D-乳酸水平显著升高;(2)与模型组比较,各给药组大鼠内毒素含量显著降低,大鼠脏器肠道菌群移位率除大黄-黄芪1:2组外,各给药组细菌移位率降低显著差异,occludin蛋白相对表达量显著升高,D-乳酸值有所降低;(3)与大黄组比较,大黄-黄芪不同剂量配比组中大黄-黄芪1:4组降低内毒素作用最为显著。结论:慢性肾衰会使大鼠肠粘膜发生损伤,肠通透性增加,血浆内毒素和肠道菌群发生移位;大黄-黄芪不同剂量配比可以改善慢性肾衰大鼠的肠粘膜损伤,降低肠粘膜通透性,使内毒素和肠道菌群移位得到抑制,其中以大黄-黄芪1:4组改善作用显著。Objective： To investigate effects of different ratios of Rhubarb and Astragale on intestinal mucosa and bacterial translocation in chronic renal failure rats. Method： The chronic renal failure rats were randomly divided into the model group, Benazepril（ 5 mg/kg）group, Rhubarb group （ 1.5 g/kg） and different ratios of Rhubarb and Astragale （ 1 ： 1, 1：2, 1：4, 1：6） （3.0,4.5,7.5, 10.5 g/kg） groups. The plasma en- dotoxine, serum D-lactate , the relative expression of ileum occludin proteins and gut bacteria translocation respectively were measured by the end point chromogenic reagent of TAL （ end-point method） , Biotin double antibody sandwich enzyme-linked immunosorbent method （ BA- ELISA）, Western blot and plate counting of bacteria method. Results： ①Compared with the sham-operated group, levels of plasma endotox- ine and gut bacteria translocation in chronic renal failure rats increased （ p 〈 0.01 ） , the relative expressions of occludin proteins reduced （ p 〈 0.01 ） and serum D-lactate increased （ P = 0. 077 ） ; ②Compared with the model group, levels of endotoxine obviously reduced （ p 〈 0.01 ） , relative expressions of occludin proteins increased （ p 〈 0. 005 ） and the rate of gut bacteria translocation reduced （ P 〈 0.05 ） except in Rhubarb and Astragale （1：2） group, the serum D-lactate slightly reduced; ③Compared with the Rhubarb group, the level of endotoxine in Rhubarb and Astragale （1：4） group obviously reduced （P 〈0.05 ）. Conclusion： ①Chronic renal failure can damage rats＇intestinal muco- sa, increased intestinal permeability, plasma endotoxin and intestinal flora translocation; ②Different ratios of Rhubarb and Astragale can suppress the intestinal mucosa injury in rats with chronic renal failure , reduce intestinal permeability, endotoxin and suppress intestinal flora translocation, among them effects of Rhubarb and Astragale （1 ：4） will be optimal.

关 键 词：大黄-黄芪 慢性肾衰竭 内毒素 细菌移位 肠粘膜损伤 D-乳酸 

分 类 号：R285.5[医药卫生—中药学]