消定膏对兔胫骨骨折愈合中TGF-β/BMPs信号通路表达的影响  

Influence of Xiaoding Ointment on expressions of TGF-β/BMPs signal pathway during rabbits tibia fracture healing

作　　者：董万涛[1,2] 辛亮[3] 宋敏[2] 冯宜蒀[1] 吕泽斌 张洁[1] 刘保健[1] 

机构地区：[1]甘肃中医药大学附属医院,兰州730020 [2]甘肃中医药大学,兰州730000 [3]四川省骨科医院,成都610000 [4]甘肃省平凉市人民医院,平凉744000

出　　处：《中药药理与临床》2017年第2期144-148,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：吴阶平医学基金会临床科研专项基金资助项目(320.6750.11061);甘肃省省青年自然科学基金计划(1208RJYA066)

摘　　要：目的:观察消定膏对兔胫骨骨折愈合中骨钙素、转化生长因子-β1(TGF-β1)、骨形态发生蛋白-2(BMP-2)、骨形态发生蛋白-7(BMP-7)不同时相表达的影响。方法:采用骨缺损法造成家兔胫骨3mm骨缺损模型,随机分为对照组和消定膏组,外敷消定膏治疗后,分别于第7天、第14天、第28天、第42天4个不同时相点每组随机抽取8只,心脏取血,ELISA法检测血清骨钙素浓度,取骨痂组织,光镜下观察病理形态学变化,免疫组化法与RT-PCR技术检测骨痂中TGF-β1、BMP-2、BMP-7蛋白表达情况。结果:局部应用0.5g/cm^2消定膏后,与对照组比较,消定膏组血清骨钙素浓度值在各个时相点均高于对照组,消定膏组骨痂出现时间早、骨痂量多、骨折愈合时间明显缩短。消定膏组BMP-2、BMP-7、TGF-β1免疫组化MOD值、RT-PCR表达量在4个不同时相点均明显高于对照组,且在术后14天左右出现峰值,明显早于对照组(28天)。结论:消定膏对兔胫骨骨折愈合的促进作用可能通过调控骨痂组织中TGF-β/BMPs信号通路有关。Objective ：To investigate the influence of Xiaoding Ointment on expressions of TGF-β1, BMP-2 and BMP-7 in different stages during rabbits tibia fracture healing. Methods：3mm bone defect model of rabbit tibia was made by bone defect method. The rabbits were randomly divided into model group and Xiaoding Ointment group. 8 experimental rabbits were randomly selected at 4 different time points （ on the 7th day, the 14th day, the 28th day and the 42nd day）. Pathomorphological changes of callus was observed under light microscope and expres- sions of TGF-β1, BMP-2 and BMP-7 in callus were detected by Immunohistochemistry and RT-PCR technique. Results：Mter topical appli- cation of Xiaoding Ointment（0. 5g/cm^2） , compared with the model group, the callus of Xiaoding group early appeared, the callus volume was much more, and the healing time was obviously shortered. The expressions of BMP-2, BMP-7 and TGF-β1 in Xiaoding Ointment group were significantly higher than those in model group at different time points （ P 〈 0.05 ）, which was significantly earlier than that in model group （28 days）. Conclusion：The promoting effect of Xiaoding Ointment on the healing of rabbits tihial fracture may be related to the regu- lation of TGF-β/BMPs signal pathway in callus tissue.

关 键 词：消定膏 骨折 转化生长因子-Β 骨形态发生蛋白 

分 类 号：R285.5[医药卫生—中药学]