稻瘟病菌中HOPS复合体生物信息学分析及MoYpt7在MoVps41突变体中的定位分析  

Bioinformatics Analysis of HOPS Complex and Expression and Subcellular Localization of MoVps41 in Magnaporthe oryzae

作　　者：张晓杰[1,2] 黄峻[2,3] 陈云云[2,3] 郑秀霞[2,3] 余盼[2,3] 朱明慧[2,3] 李源[2,3] 胡燕佩 张冬梅[2,3] 王宗华[2,3] 

机构地区：[1]福建农林大学生命科学学院,福州350002 [2]福建农林大学生物农药与化学生物学教育部重点实验室,福州350002 [3]福建农林大学植物保护学院,福州350002

出　　处：《分子植物育种》2017年第5期1762-1773,共12页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31070124)资助

摘　　要：据报道,酵母中HOPS复合体参与囊泡融合及运输。本研究分别以酿酒酵母HOPS复合体各亚基氨基酸序列,通过BLASTp在稻瘟病菌数据库中搜索得到了6个酿酒酵母HOPS复合体各亚基的同源蛋白,我们通过生物信息学方法对稻瘟病菌6个HOPS复合体亚基的同源蛋白的理化特性、蛋白质结构域和进化关系进行了分析。基于稻瘟病菌98-06菌丝阶段和孢子侵染水稻CO39不同阶段转录组测序数据,分别选取稻瘟病菌内吞和外泌途径相关的基因,制作了表达模式图。此外,MoYpt7蛋白在其假定下游效应因子ΔMoVps41突变体中的定位研究表明MoVps41可能影响了液泡融合。It is reported that HOPS complex is involved in the fusion and trafficking of vacuoles in the yeast. Six homolog proteins of HOPS complex subunits were identified in Magnaporthe oryzae by BLASTp respectively using 6 amino acid sequences of HOPS complex subunits in Saccharomyces cerevsiae. We analyzed the physicochemical properties, functional domains and phylogenetic relationships of six HOPS subunit homologs in M. oryzae by bioinformatic methods. Based on the transcriptome sequencing data collected from different mycelial and infection stages ofM. oryzae to rice CO39, we conducted the distinct expression patterns of endocytosis-associated genes and secretion-related genes in M. oryzae. Furthermore, the subcellular localization of MoYpt7 in MoVps41 deletion mutant and its putative downstream effector showed that MoVps41 may be involved in vesicle fusion in M. oryzae.

关 键 词：稻瘟病菌 内吞 外泌 MoYpt7 MoVps41 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]