鸡蛋花组织培养与快速繁殖  

Tissue Culture and Rapid Propagation of Plumeria rubra L.

作　　者：李克烈[1] 李志英 王荣香[1] 

机构地区：[1]中国热带农业科学院作物品种资源研究所,农业部华南作物基因资源与种质创制重点实验室,海南省热带作物种质资源遗传改良与创新重点实验室,国家种质资源热带作物中期保存库,儋州571737

出　　处：《分子植物育种》2017年第5期1881-1886,共6页Molecular Plant Breeding

基　　金：物种资源保护(B650);中央级科研院所基本科研业务费专项(1630032012018;1630032016007);引进国际先进农业科学技术项目(2015-Z22)共同资助

摘　　要：以鸡蛋花幼嫩带芽茎段为外植体,通过对初代启动培养、增殖及生根培养方案的筛选,建立了鸡蛋花的组织培养和快速繁殖体系。结果表明,最适启动培养基为MS+6-BA 0.5 mg/L+NAA 0.02 mg/L,外植体诱导萌发率达83.33%;培养基为MS+6-BA 3.0 mg/L+NAA 0.5 mg/L,培养基MS+6-BA 2.00 mg/L+NAA 0.05 mg/L适宜继代增殖,30 d的增殖系数为8.73;生根最适培养基为1/2 MS+IBA 0.2 mg/L+NAA 1.0 mg/L,生根率达98.1%。因此,本研究为鸡蛋花培养育苗与品种改良提供依据。In this research, we took the healthy tender stem segments of P. rubra L. as explants. After the media for axillary bud induction, subculture and rooting culture were selected, the tissue culture and rapid propagation system of P. rubra L. was established. The results showed that the optimal medium for axillary bud induction was MS ＋6-BA 0.5 mg/L ＋NAA 0.02 mg/L, with an induction rate of 83.33%. The optimal subculture and proliferation media were both MS＋6-BA 3.0 mg/L＋NAA 0.5 mg/L. The propagation coefficient was 8.73 every 30 days. The optimal rooting medium was 1/2 MS＋IBA 0.2 mg/L＋NAA 1.0 mg/L, with a rooting rate of 98.1%. This study provided the basis for the breeding and variety improvement ofP. rubra L..

关 键 词：鸡蛋花 茎段 组织培养 快速繁殖 

分 类 号：S685.99[农业科学—观赏园艺]