三角梅无菌播种快速繁殖技术  

Seeds Sterile Rapid Propagation of Bougainvillea glabra

作　　者：潘梅[1] 符瑞侃 杨珺[1] 戚华沙[1] 黄赛[1] 姜殿强[1] 王景飞[1] 云勇 

机构地区：[1]海南省农业科学院热带园艺研究所,海口571100

出　　处：《分子植物育种》2017年第5期1894-1899,共6页Molecular Plant Breeding

基　　金：海南省重大科技项目(ZDZX2013012)资助

摘　　要：以种子作为外植体,通过比较植物生长调节剂种类和浓度配比,建立三角梅的组培快繁技术。结果表明:种子在MS+蔗糖3%培养基中萌发;培养基MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+蔗糖3%有利于丛生芽的生长发育,30 d的增殖系数达到5.22;培养基MS+NAA 1.5 mg/L+蔗糖3%适宜诱导生根,生根率为95.56%,平均每株生根6.39条。Using the seeds as explants, the techniques of tissue culture and rapid propagation of Bougainvillea glabra were studied by comparing with plant growth regulator type and concentration ratio. The results showed that seeds sprouted buds in the medium MS ＋sucrose 3%; MS＋6-BA 1.0 mg/L＋NAA 0.2 mg/L＋sucrose 3% could effectively induce fasciculate buds, the propagation coefficient was 5.22 every 30 days; the most optimum rooting medium was MS＋NAA1.5 mg/L＋sucrose 3%, the rooting rate was 95.56% and the average root number was 6.39.

关 键 词：三角梅 种子 丛生芽 快速繁殖 

分 类 号：S685.99[农业科学—观赏园艺]