二冬膏含药血清对人肺癌A549细胞的抑制效应初探  

Preliminary investigation of the inhibitory effect of Erdong Gao on human lung cancer A549 cells

作　　者：江闰德[1] 张志雄[2] 林莉[2] 邓飞[2] 

机构地区：[1]江西中医药大学生命科学学院,南昌330004 [2]南方医科大学深圳医院病理科,深圳518110

出　　处：《北京中医药》2017年第4期330-333,共4页Beijing Journal of Traditional Chinese Medicine

基　　金：江西省教育厅科技项目(GJJ14604);江西中医药大学重点学科青年基金项目(2013jzzdxk029);深圳市宝安区医疗卫生科研项目(2016CX316)

摘　　要：目的探讨二冬膏含药血清对人肺癌A549细胞的抑制效应。方法采用中药血清药理学方法,选择高、中、低剂量的二冬膏水溶液以灌胃方式给药,获取二冬膏含药血清,以空白血清为对照,干预A549细胞,应用MTT法、划痕实验法及Transwell侵袭实验法检测二冬膏含药血清对A549细胞增殖、迁移及侵袭能力的影响。结果二冬膏高、中、低剂量组干预A549细胞24 h后均可不同程度抑制肺腺癌A549细胞增殖,差异有统计学意义(P<0.05),并呈时间、剂量依赖性,以二冬膏中剂量组抑制作用最明显;二冬膏高、中、低剂量组A549细胞的迁移距离较空白组缩短,差异有统计学意义(P<0.05),中剂量组细胞迁移距离最短;二冬膏高、中、低剂量组均能降低A549细胞的侵袭能力,差异有统计学意义(P<0.05),中剂量组侵袭过膜的细胞数最少。结论二冬膏含药血清能抑制肺癌A549细胞增殖、迁移及侵袭能力,中剂量抑制作用最明显。Objective To investigate the inhibitory effect of Erdong Gao contained serum on human lung cancer A549 cells. Methods The serum containing Erdong Gao were obtained by the serum pharmacological method of traditional Chinese medicine （TCM） with the high, medium and low dosage of Erdong Gao given with gavage. The blank serum was used as the control and AS49 cells were intervened by Erdong Gao. MTT assay, scratch test and Transwell invasion assay were used to detect the effect of Erdong Gao contained serum on the proliferation, migration and invasion of A549 cells. Results The proliferation of A549 cells were inhibited differently by the intervention of high, middle and low dosage of Erdong Gao after 24h, and the differences were statistically significant （ P 〈 0. 05 ）. The inhibitory effect was depended on the dosage of Erdong Gao and the times of intervention with the effect of middle dosage the most obvious. The migration distance of A549 cells treated with different doses of Erdong Gao was significantly shorter than that of the blank control group nd the differences were statistically significant （ P 〈 0. 05 ） with the distance of middle dosage group the shortest. The invasive ability of A549 treated with different doses of Erdong Gao were decreased, the differences were statistically significant （P 〈 0. 05 ）, and the number of invasion cells was least in the middle dosage group. Conclusion Erdong Gao contained serum could inhibit the proliferation, migration and invasion of lung cancer A549 cells. The inhibitory effect is the most obvious in the medium dosage group.

关 键 词：二冬膏 肺癌 A549细胞 生物学行为 

分 类 号：R285[医药卫生—中药学]