利用90k芯片技术进行小麦穗部性状QTL定位  

QTL Mapping for Spike Traits of Wheat Using 90k Chip Technology

作　　者：武炳瑾 简俊涛 张德强[1] 马文洁[1] 冯洁[1] 崔紫霞 张传量 孙道杰[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《作物学报》2017年第7期1087-1095,共9页Acta Agronomica Sinica

基　　金：国家重点基础研究计划(973计划)项目(2014CB138100);陕西省自然科学基金项目(2015JM3094);陕西省重点科技创新团队项目(2014KCT-25)资助;supported by the National Key Basic Research Program of China(2014CB138100);the Natural Science Foundation of Shaanxi Province(2015JM3094);the Key Scientific and Technological Innovation Team of Shaanxi Province(2014KCT-25)

摘　　要：小麦穗部性状与产量密切相关,挖掘穗部性状基因及其关联分子标记具有重要意义。本研究以周8425B?小偃81衍生的RIL群体(F8)为材料,利用90k芯片标记构建的高密度遗传图谱对3个环境下的穗长、小穗数、不育小穗数、穗粒数、千粒重进行QTL定位。共检测到19条染色体上的71个QTL,变异解释率(PVE)范围为2.10%~45.25%,其中37个位点为主效QTL(PVE>10%)。QSl.nafu-6A.2(穗长)、QSl.nafu-7A(穗长)、QSsn.nafu-2A.1(不育小穗数)、QSsn.nafu-2D(不育小穗数)和QGns.nafu-2B(穗粒数)在多个环境中被检测到,且LOD>10,PVE>20%。位于同一个基因簇中的QSl.nafu-6A.2(穗长)、QGns.nafu-6A(穗粒数)和QTgw.nafu-6A(千粒重)在多个环境中被检测到,且与已报道的相关位点位置相同或相近,在分子标记辅助育种中具有较大参考价值。Spike traits are important to grain yield in wheat. Molecular markers associated with genes/QTLs controlling spiketraits are highly valuable to marker-assisted breeding. A recombinant inbred line （F8） population derived from Zhou 8425B？Xiaoyan 81 were evaluated in three environments, and QTLs for spike length, spikelet number per spike, sterile spikelet number,grain number per spike and thousand-grain weigh were mapped into a high-density genetic map built by 90k chip. A total of 71QTLs were located on 19 chromosomes, and the phenotype variation explained （PVE） by a single locus ranged from 2.10% to45.25%. Thirty-seven loci were considered as main-effect QTLs owing to the PVE larger than 10%. QTLs QSl.nafu-6A.2 for spikelength, QSl.nafu-7A for spike length, QSsn.nafu-2A.1 for sterile spikelet number, QSsn.nafu-2D for sterile spikelet number andQGns.nafu-2B for grain number per spike were identified repeatedly in different environments with the LOD value higher than 10and PVE larger than 20%. QSl.nafu-6A.2 for spike length, QGns.nafu-6A for grain number per spike and QTgw.nafu-6A for thousand-grain weight were mapped in a cluster on chromosome 6A and might be applicable in marker-assisted selection because theyhave been detected in multiple environments and close to the loci reported.

关 键 词：小麦 穗部性状 90k基因芯片 QTL定位 

分 类 号：S512.1[农业科学—作物学]