绿豆分子遗传图谱构建及若干农艺性状的QTL定位分析  

Construction of Genetic Map and Identification of QTLs Related to Agronomic Traits in Mung Bean

作　　者：王建花[1,2,3] 张耀文[3] 程须珍[2] 王丽侠[2] 

机构地区：[1]山西大学,山西太原030006 [2]中国农业科学院作物科学研究所,北京100081 [3]山西省农业科学院作物科学研究所,山西太原030031

出　　处：《作物学报》2017年第7期1096-1102,共7页Acta Agronomica Sinica

基　　金：国家现代农业产业技术体系建设专项(CARS-09);中国农业科学院农业科技创新工程资助~~

摘　　要：利用花叶1号×紫茎1号杂交后代衍生的208个F2家系组建群体,构建含有95个SSR标记位点的遗传连锁图谱,该图谱包含11个连锁群,全长1457.47 c M,标记平均间距为15.34 c M。利用复合区间作图法,对株高、幼茎色、主茎色、生长习性、结荚习性、复叶叶形和成熟叶色等农艺性状进行QTL分析,分别检测到与株高、幼茎色、主茎色、复叶叶形有关的QTL各1个,贡献率在8.49%~66.64%之间;与结荚习性有关的QTL3个,贡献率在60.32%~80.36%之间;与成熟叶色有关QTL 4个,贡献率在69.06%~87.35%之间;与生长习性有关的QTL数量最多,共26个,贡献率在58.32%~99.51%之间。上述QTL主要分布在LG1、LG2、LG4、LG8和LG10连锁群,其中LG1最少,仅检测到生长习性的1个QTL,LG4最多,包含了幼茎色、主茎色、结荚习性、生长习性、复叶叶形、成熟期叶色6个农艺性状的15个QTL;这些QTL既可以应用于绿豆育种的分子标记辅助选择,也对深入研究这些性状的遗传奠定了基础。Two hundreds and eight individuals of F2 population, derived from a cross between two mung bean genotypes （Huaye 1and Zijing 1） were used to construct genetic map, and to identify QTLs related to important agronomic traits. This genetic mapcontained 11 linkage groups with a total length of 1457.47 cM and an average interval of 15.34 cM. QTLs mapping was conductedfor plant height, young stem color, main stem color, growth habit, podding habit, trilobate leaf shape and mature leaf colorusing composite interval mapping method. Only one QTL for each trait was detected including plant height, young stem color,main stem color and trilobate leaf shape, and with a contribution ranging from 8.49% to 66.64%. Three QTLs with high contributionrates from 60.32% to 80.36% were identified for the trait of pod habit in mung bean. Four QTLs related to mature leaf colorshowed at contribution rate from 69.06% to 87.35%. There were 26 QTLs related to growth habit, the most of the tested QTLs,with a contribution rate each from 58.32% to 99.51%. The present QTLs for seven agronomic traits distributed on LG1, LG2, LG4,LG8, and LG10, respectively, could be used in molecular breeding based on marker-assisted selection in mung bean, and also laya foundation for further study of the inheritance of these traits.

关 键 词：绿豆 连锁遗传图谱 SSR标记 复合区间作图法 QTL 贡献率 

分 类 号：S522[农业科学—作物学]