新生儿感染不同病原体后体内相关免疫细胞及细胞因子的变化  

Changes of immune cells and cytokines in neonates infected by different pathogens

作　　者：彭富栋[1] 许玉静[1] 李洪双[1] 单玉凤 王帮[1] 

机构地区：[1]聊城市第二人民医院,山东聊城252601

出　　处：《中国妇幼保健》2017年第11期2369-2371,共3页Maternal and Child Health Care of China

摘　　要：目的探讨新生儿感染不同病原体后体内相关免疫细胞及其细胞因子的变化。方法选择2011年3月-2014年8月在聊城市第二人民医院新生儿监护室治疗的160例患儿作为研究对象,其中感染细菌组50例,感染病毒组50例,60例ABO溶血病患儿作为对照组,3组患儿均采用流式细胞仪检测静脉血CD_4^+T淋巴细胞因子和T淋巴细胞亚群的变化,并进行比较。结果细菌组IL-4水平与病毒组、对照组比较,差异均有统计学意义(t=2.814,P<0.01;t=-3.715,P<0.01),病毒组与对照组间IL-4水平比较,差异无统计学意义(t=-5.98,P>0.05);3组新生儿间的CD_3^+、CD_4^+、CD_8^+、CD_(19)^+、CD_4^+/CD_8^+及NK细胞阳性率比较,差异均有统计学意义(均P<0.05);细菌组和病毒组的CD_3^+、CD_4^+和CD_4^+/CD_8^+水平比较,差异均有统计学意义(t=-5.977,t=-1.175,P<0.05;t=-3.942,t=-3.562,P<0.05);细菌组和病毒组CD_(19)^+水平比较,差异有统计学意义(t=10.386,t=13.389,P<0.05);细菌组与病毒组间CD_3^+、CD_4^+、CD_(19)^+和CD_4^+/CD_8^+水平比较,差异均无统计学意义(P>0.05);病毒组NK细胞阳性率比较,差异均有统计学意义(t=-2.28,t=-2.28,P<0.05),细菌组与对照组间NK细胞阳性率比较,差异无统计学意义(t=0.717,P>0.05)。结论在新生儿期的任何外源性病原体感染都会导致T淋巴细胞亚群受到抑制,相对来说,Th2型细胞受到的抑制比Th1型更加严重,NK细胞对病毒感染的抵抗力要强于细菌感染。Objective To explore the changes of immune cells and cytokines in neonates infected by different pathogens. Methods A total of 160 neonates treated in neonatal intensive care unit of the hospital from March 2011 to August 2014 were selected as research object, then they were divided into bacterium group （50 neonates with bacterial infection）, virus group （50 neonates with viral infection）, and control group （60 neonates with ABO hemolytic disease） . Flow cytometry was used to detect the changes of CD4^＋T lymphocyte factors and T lymphocyte subsets in venous blood in the three groups, then the results were compared. Results There were statistically significant differences in IL-4 level between bacterium group and virus group, control group, respectively （t = 2. 8114, P〈0. 01 ; t =-3. 715, P〈0. 01 ）. There was no statistically significant difference in IL-4 level between virus group and control group （ t = -5.98, P〉0. 05 ） . There were statistically significant differences in positive rates of CD3^＋ , CD4^＋ , CD8^＋ , CD19^＋ , CD4^＋/CD8^＋, and NK cells among the three groups （all P〈 0. 05 ） . There were statistically significant differences in CD3^＋ , CD4 ＋ , and CD4^＋/CD8^＋ levels between bacterium group and virus group （t =-5. 977, t =-1. 175, P〈0. 05; t =-3. 942, t =-3. 562, P〈0. 05 ） . There was statistically significant difference in CD19^＋ level between bacterium group and virus group （ t = 10. 386, t = 13. 389, P〈0. 05 ） . There was no statistically significant difference in CD3^＋, CD4 ^＋, CD19^ ＋, and CD4^ ＋/CD8^ ＋ levels between bacterium group and virus group （P〉0. 05 ） . There was statistically significant difference in positive rate of NK cells between bacterium group and virus group （t=-2. 28, P〈0. 05 ） . There was no statistically significant difference in positive rate of NK cells between bacterium group and control group （t = 0. 717, P〉0. 05 ） .Condusion During neonatal period, any exogenous pathogens wi

关 键 词：新生儿 感染 CD4^+T淋巴细胞因子 T淋巴细胞亚群 

分 类 号：R725[医药卫生—儿科]