超促排卵对小鼠胚胎发育及输卵管中IL-6表达的影响  

Effect of controlled ovarian hyperstimulation on the development of embryos and salpingian IL-6 expression in mice

作　　者：尉春华[1] 胡彩妮 姚元庆[1] 

机构地区：[1]解放军总医院妇产科,北京100853 [2]武警甘肃总队医院妇产科,甘肃兰州730050

出　　处：《武警后勤学院学报（医学版）》2017年第3期198-201,共4页Journal of Logistics University of PAP（Medical Sciences）

基　　金：中国博士后科学基金资助项目(2013M542519)

摘　　要：【目的】探讨超排卵对小鼠受精卵发育到囊胚及受精卵期输卵管中白细胞介素-6(Interleukin-6,IL-6)表达的影响。【方法】90只雌性ICR小鼠采用随机数字表法分为对照组(60只)和超排组(30只),收集受精卵体外培养3 d,显微镜下观察两细胞、四细胞及囊胚形成率。收集对照组和超排组小鼠受精后0.5 d(days post coitum,dpc)的输卵管,应用免疫荧光共聚焦法检测其IL-6的表达。【结果】与对照组相比,超排组两细胞形成率及囊胚形成率降低,二者差异具有统计学意义(P<0.05);免疫荧光共聚焦结果显示0.5 dpc超排组输卵管中IL-6表达多于对照组。【结论】控制性超排卵能够增加受精卵期输卵管中IL-6的表达,可能与其保障受精卵发育有关。【Objective】To explore the effect of controlled ovarian hyperstimulation on the development of zygote to blastocyst and the salpingian IL-6 expression during the zygote stage in mice.【Methods】A total of 90 female ICR mice were randomly divided into control group（n=60） and superovulated group（n=30） by random number table.Mouse zygotes were collected and cultured in vitro for 3d.The formation rates of 2 cells,4 cells and blastocyst were observed under microscope.The 0.5dpc salpinges in pregnant mice were collected from the control group and superovulated group,and the expression of IL-6 in fallopian tubes was detected by confocal immunofluorescence method.【Results】The rates of 2-cell formation and blastocyst formation in the superovulated group were significantly lower than those in the control group（P〈0.05）.The results of confocal immunofluorescence method,showed that the salpingian IL-6 expression in 0.5dpc superovulated group was obviously higher than that in the control group.【Conclusion】Controlled ovarian hyperstimulation can increase the salpingian IL-6 expression at zygote stage,which may relate to supporting the development of zygotes.

关 键 词：超排卵 小鼠 受精卵 白细胞介素-6 输卵管 

分 类 号：R392[医药卫生—免疫学]