Discovery of lung squamous carcinoma biomarkers by profiling the plasma peptide with LC/MS/MS  

作　　者：Yu Liu Xiao-Hong Xun Jian-Ming Yi Yang Xiang Jie Hua 

机构地区：[1]College of Chemistry and Chemical Engineering,Hunan Institute of Science and Technology,Yueyang 414006,China

出　　处：《Chinese Chemical Letters》2017年第5期1093-1098,共6页中国化学快报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(No.21172067);Science and Technology Project of Hunan Province(Nos.2013SK2015,2014SK3017);Scientific Research Key Fund of Hunan Provincal Education Department(No.13A032)

摘　　要：Biomarkers can be used for the screening and clinical diagnosis of cancer,and peptidomics approach has been proven successful in the research of biomarkers.To develop better peptidomic technologies for fast,accurate,and reliable detection of peptides biomarkers for lung cancer,we have improved the procedures of blood collection to minimize the degradation of the blood proteins and optimize the extraction of peptidome peptides from plasma samples based on acetonitrile precipitation associated with size exclusion chromatography（SEC）.Studies show that squamous cell carcinomas are found to express CAGEl,SPAT9 and TEX28 genes at significantly higher rates,and the results suggest that as tumors progress,the level of CAGEl,SPAT9 and TEX28 genes are likely to increase and lead to immunization.This suggests a potentially important therapeutic method for cancer testis-based cancer vaccines.Biomarkers can be used for the screening and clinical diagnosis of cancer,and peptidomics approach has been proven successful in the research of biomarkers.To develop better peptidomic technologies for fast,accurate,and reliable detection of peptides biomarkers for lung cancer,we have improved the procedures of blood collection to minimize the degradation of the blood proteins and optimize the extraction of peptidome peptides from plasma samples based on acetonitrile precipitation associated with size exclusion chromatography（SEC）.Studies show that squamous cell carcinomas are found to express CAGEl,SPAT9 and TEX28 genes at significantly higher rates,and the results suggest that as tumors progress,the level of CAGEl,SPAT9 and TEX28 genes are likely to increase and lead to immunization.This suggests a potentially important therapeutic method for cancer testis-based cancer vaccines.

关 键 词：Lung cancer Lung squamous carcinoma Biomarkers Cancer testis antigen Peptidomics 

分 类 号：O657.63[理学—分析化学] R734.2[理学—化学]