UPLC指纹图谱结合模式识别分析不同产地生/制何首乌  

Analyzing crude/processed root of Polygonum multiflorum from different habitats by UPLC fingerprint and mode identification methods

作　　者：肖榕[1,2] 林艳[1,2] 雷思敏[1,2] 章莹[1,2] 黄杰[1,2] 夏伯候[1,2] 李春[3] 廖端芳[1,2] 吴萍[1,2] 林丽美[1,2] 

机构地区：[1]湖南中医药大学药学院,湖南长沙410208 [2]湘产大宗药材品质评价湖南省重点实验室,湖南长沙410208 [3]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2017年第12期2305-2310,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金青年项目(21302229);中医药行业科研专项(201507002-2;201507002-3);长沙市科技计划重点项目(k1406030-31);湖南省十三五重点学科药学学科项目

摘　　要：采用超高效液相色谱(UPLC),建立不同产地生/制何首乌二苯乙烯苷(TSG)的含量测定方法和指纹图谱。大孔树脂柱处理各样品,UPLC分析,Waters ACQUITY UPLC@BEH C18色谱柱(2.1 mm×50 mm,1.7μm),乙腈-0.5%甲酸溶液梯度洗脱,流速0.3 mL·min^(-1),波长254 nm,柱温(25±5)℃。结果发现大孔树脂分离后药材二苯乙烯苷含量均在25.0%以上;成功建立不同产地生/制何首乌药材UPLC指纹图谱;标定了17个色谱峰,聚类分析不能完全将生/制首乌分开,而主成分分析可明显地将其分为两类;二苯乙烯苷是两类何首乌自变量投影重要性指标(VIP)差异最大的组分。前处理方法可获得高含量的二苯乙烯苷,测定方法简单,灵敏,可靠,可用于生/制何首乌的快速鉴别,可作为何首乌药材整体质量控制的方法之一。To establish a content determination method for 2,3,5,4＇-tetrahydroxy stilbene-2-O-β--D-glucoside （TSG） of the crude/ processed root of Polygonum multiflorum from different habitats in China and set up the fingerprint by using UPLC. Various samples were pretreated by macro-porous resin. Then UPLC analysis was performed on Waters ACQUITY UPLC@ BEH C18 chromatographic col- umn （2. 1 mm×50mm,1.7μm） at （ 25 ± 5 ） ℃. A binary gradient elution system was composed of aeetonitrile （ phase A） and O. 5% acetic acid solution （phase B）. Detection was performed at the wavelength of 254 nm, and the mobile flow rate was set at 0. 3 mL min-1. Results showed that the yield of extraction of the 2,3 ,5 ,4＇-tetrahydroxy stilbene-2-O-β-D-glucoside from root of P. multi- florum was all over 25.0% after macro-porous resin separation; an exclusive UPLC fingerprint method of the crude/processed root ofP. multiflorum from different habitats was successfully set up and 17 chromatographic peaks were calibrated. Cluster analysis can not entirely distinguish the crude one from the processed one, while principal component analysis absolutely can. 2,3,5,4＇-tetrahydroxy stilbene-2-O-β-D-glucoside is the composition that has largest differences in variable importance in projection （VIP） between crude and processed root of P. multiflorum. The separating method can gain high-purity 2,3,5,4＇-tetrahydroxy stilbene-2-O-β--D-glucoside, and the determination method is simple, sensitive, reliable and can be used in fast identifying the crude/processed root of P. multiflorum or as a method for overall quality control of root of P. multiflorum.

关 键 词：何首乌 二苯乙烯苷 UPLC指纹图谱 聚类分析 主成分分析 

分 类 号：O657.72[理学—分析化学] R284.1[理学—化学]