冬凌草甲素诱导三阴乳腺癌MDA-MB-231细胞凋亡及对细胞内活性氧水平的影响  

Effect of oridonin on apoptosis and intracellular reactive oxygen species level in triple-negative breast cancer MDA-MB-231 cells

作　　者：齐琦[1] 张配[2] 李其响 潘琼[2] 郑海伦[3] 赵素容[2] 

机构地区：[1]蚌埠医学院基础医学部,安徽蚌埠233030 [2]蚌埠医学院药学系,安徽蚌埠233030 [3]蚌埠医学院第一附属医院,安徽蚌埠233004

出　　处：《中国中药杂志》2017年第12期2361-2365,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81372899;81603155);安徽省高等学校自然科学研究重点项目(KJ2016A486;KJ2016A869;KJ2015A177)

摘　　要：冬凌草甲素是从中药冬凌草Rabdosia rubescens中分离得到的一种贝壳杉烯二萜类的天然有机化合物,具有抗炎、抗菌、抗肿瘤等多种生物活性。该实验观察冬凌草甲素对三阴乳腺癌MDA-MB-231细胞凋亡的影响及其可能的作用机制。采用MTT法检测冬凌草甲素对MDA-MB-231细胞的增殖抑制作用,PI单染、Annexin V-FITC/PI双染流式细胞术分析冬凌草甲素对MDA-MB-231细胞凋亡的影响,活性氧(ROS)试剂盒检测细胞内ROS水平的变化,Western blot分析PARP,Bcl-2,caspase-3蛋白的表达情况。结果表明,冬凌草甲素对乳腺癌MDA-MB-231细胞具有明显的凋亡诱导作用,显著升高细胞内的ROS水平,下调抗凋亡蛋白Bcl-2的表达,并促进caspase-3及其底物PARP的切割。提示,冬凌草甲素诱导MDA-MB-231细胞凋亡的作用可能与升高细胞内ROS水平、下调Bcl-2的表达以及激活caspase-3相关。Oridonin, which is an ent-kaurene diterpenoid isolated from traditional Chinese medicine Rabdosia rubescens, displays various bioactivities, including anti-inflammation, anti-bacteria and anti-tumor. This study aimed to investigate the effect of oridonin on apoptosis of triple-negative breast cancer MDA-MB-231 cells and its underlying mechanisms. The inhibitory effect of oridonin on prolif- eration of MDA-MB-231 cells was measured by MTT assay; Apoptosis was analyzed by flow cytometry with PI staining and Annexin V- FITC/PI staining; Intracellular reactive oxygen species （ROS） level was determined by ROS detection kit, and expressions of PARP, Bcl-2, caspase-3 were analyzed by Western blot. The results showed that oridonin exhibited a significant effect in inducing apoptosis of MDA-MB-231 cells, enhancing intracellular ROS level, down-regulating expression of Bcl-2 protein, and promoting cleavage of caspase-3 and its substrate PARP. These results indicated that the apoptosis-inducing effect of oridonin on MDA-MB-231 cells might be correlated with increase of intracellular ROS level, down-regulation of Bcl-2 protein and activation of caspase-3.

关 键 词：乳腺癌 冬凌草甲素 细胞凋亡 活性氧 

分 类 号：R285[医药卫生—中药学]