消银解毒颗粒对Jurkat细胞异常增殖模型的影响  

Effect of Xiaoyin Jiedu Granules(消银解毒颗粒) on Model of Jurkat Cell Abnormal Proliferation

作　　者：张润田[1] 陈曦[2] 霍春波[1] 段行武[1] 陈兵[1] 李玲玲[1] 

机构地区：[1]北京中医药大学东直门医院,北京市东城区海运仓5号100700 [2]北京中医药大学东直门医院东区

出　　处：《中医杂志》2017年第12期1049-1052,共4页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81273755;81403403;81403404);2016年东直门医院青苗人才培养计划(DZMYS-201603);北京中医药大学杰出青年人才项目(2016-JYB-XJQ005)

摘　　要：目的探讨消银解毒颗粒治疗银屑病的可能作用机制。方法以植物血凝素(PHA)和白细胞介素6(IL-6)共同诱导Jurkat细胞模拟银屑病T细胞异常增殖的病理模型,制备择取最优效药理血清浓度作用于该细胞模型,采用细胞活性试剂盒(CCK-8)法检测细胞活力,用流式细胞术检测细胞凋亡。结果当药理血清浓度为20%时,消银解毒颗粒组、凉血方组细胞抑制率显著高于解毒方组、阿维A组、蒸馏水组(P<0.01);消银解毒颗粒组细胞早期凋亡率最高,高于凉血方组、解毒方组、阿维A组、蒸馏水组(P<0.05或P<0.01)。结论消银解毒颗粒及其拆方凉血方、解毒方对银屑病T细胞异常增值的炎症病理模型均有抑制作用,且消银解毒颗粒和凉血方的抑制效果明显优于阿维A。Objective To explore the possible mechanism of Xiaoyin Jiedu Granules （消银解毒颗粒） in the treat- ment of psoriasis. Methods The Jurkat cell was administered by both phytohemagglutinin （PHA） and interleukin 6 （IL-6） to induce the model of psoriasis T cell abnormal proliferation. The optimal pharmaceutical serum concentration was made and selected to be applied to the cell model. The cell viability was measured with cell viability kit （CCK-8）, and the apoptosis was detected with flow cytometry. Results When the pharmaceutical serum concentration was 20% , the inhibition rates in the Xiaoyin Jiedu Granules group and Liangxuefang group were significantly higher than that in the Jiedufang group, Acitretin group and distilled water group （ P 〈 0. 01 ） ; the Xiaoyin Jiedu Granules group had the highest early apoptosis rate, which was significantly higher than that in Liangxuefang group, Jiedufang group, Acitretin group and distilled water group （ P 〈 0. 05 or P 〈 0. 01 ）. Conclusion Xiaoyin Jiedu Granules and its decomposed recipes could inhibit the inflammatory pathological model of psoriasis T cell abnormal proliferation, and the inhibitory effects of Xiaoyin Jiedu Granules and Liangxuefang seem better than Acitretin.

关 键 词：银屑病 消银解毒颗粒 T细胞 异常增殖 

分 类 号：R285[医药卫生—中药学]