紫外-可见分光光度法测定紫草油中羟基萘醌总色素的含量  

Content Determination of Hydroxynaphthoquinone Pigments in Zicao Oil by Ultraviolet-Visable Spectrophotometry

作　　者：苗颖[1] 李英男[2] 李栋[1] 梁莹莹[1] 张淑君[1] 陆进[1] 赫军[1] 马秉智[1] 杨丽 

机构地区：[1]中日友好医院药学部,北京100029 [2]清华大学医院药剂科,北京100084 [3]北京市食品药品监督管理局注册处,北京100053

出　　处：《中国医院用药评价与分析》2017年第5期587-588,共2页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：首都卫生发展科研专项项目(No.首发2011-2008-01);北京市医疗机构制剂标准评价体系及标准提高研究(No.Z121100000312005)

摘　　要：目的:建立紫外-可见分光光度法测定紫草油中羟基萘醌总色素含量的方法。方法:羟基萘醌总色素的含量以左旋紫草素计,溶剂为乙醇,检测波长为516 nm。结果:左旋紫草素质量浓度在8.08~40.40μg/ml范围内与吸光度呈良好的线性关系(r=0.999 9,n=5),平均加样回收率为97.97%(RSD=1.70%,n=5)。结论:该方法简便、准确,回收率与精密度高,重现性好,可用于紫草油中羟基萘醌总色素含量的测定。Objective：To establish a method for content determination of hydroxynaphthoquinone pigments in zicao oil by ultraviolet-visable spectrophotometry.METHODS： Content of hydroxynaphthoquinone pigments was counted in L-shikonin.Methanol was used as solvent and the wavelength was set at 516 nm.RESULTS： The concentration of L-shikonin showed a good linear relationship with absorbance in the range of 8.08 μg/ml-40.40 μg/ml（r=0.999 9,n=5）.The average recovery was 97.97%（RSD=1.70%,n=5）.CONCLUSIONS： This method is simple,precise and replicable resulting in high recovery and accuracy,which can be used to determine the content of hydroxynaphthoquinone pigments in zicao oil.

关 键 词：紫外-可见分光光度法 紫草油 左旋紫草素 羟基萘醌总色素 含量测定 

分 类 号：R927.2[医药卫生—药学]