HPLC法同时测定祛瘀止痛合剂中6种成分的含量  

Simultaneous Determination of 6 Components in Quyu Zhitong Mixture by HPLC

作　　者：李洁环[1,2] 王洛临 郭鸣[1,2] 徐文杰 陈雪婷 李智勇 

机构地区：[1]广州中医药大学附属广东第二中医院,广州510405 [2]广东省中医药工程技术研究院/广东省中医药研究开发重点实验室,广州510095

出　　处：《中国药房》2017年第18期2557-2560,共4页China Pharmacy

基　　金：广东省省级科技计划项目(No.2013B040200040)

摘　　要：目的:建立同时测定祛瘀止痛合剂中芍药苷、柚皮苷、新橙皮苷、三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1含量的方法。方法:采用高效液相色谱法。色谱柱为Waters Xbridge C18,流动相为0.1%磷酸-乙腈(梯度洗脱),流速为1.0 m L/min,检测波长为203 nm,柱温为20℃,进样量为5μL。结果:芍药苷、柚皮苷、新橙皮苷、三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1检测进样量线性范围分别为0.273 4~2.734μg(r=0.999 9)、0.119 1~1.191μg(r=0.999 9)、0.081 5~0.815μg(r=0.999 9)、0.622 8~6.228μg(r=0.999 9)、0.807 2~8.072μg(r=0.999 9)、1.036 4~10.364μg(r=0.999 9);定量限分别为0.082 0、0.029 8、0.028 5、0.436 0、0.403 6、0.310 9μg,检测限分别为0.027 9、0.009 5、0.010 2、0.124 6、0.121 1、0.093 3μg;精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为97.85%~100.34%(RSD=0.81%,n=6)、98.14%~101.22%(RSD=1.09%,n=6)、98.42%~102.15%(RSD=1.29%,n=6)、97.77%~100.25%(RSD=0.96%,n=6)、97.32%~99.53%(RSD=0.81%,n=6)、98.28%~101.51%(RSD=1.11%,n=6)。结论:该方法简便快速、稳定可行、重复性好,可用于祛瘀止痛合剂中6种成分含量的同时测定。OBJECTIVE： To establish a method for simultaneous determination of paeoniflorin, naringin, neohesperidin, notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rbl in Quyu zhitong mixture. METHODS： HPLC method was adopted. The determination was performed on Waters Xbridge C18 column with mobile phase consisted of 0.1% phosphoric acid-acetonitrile （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 203 nm, and column temperature was 20℃. The sample size was 5 μL. RESULTS： The linear ranges ofpaeoniflorin, naringin, neohesperidin, notoginsenoside Rt, ginsenoside Rgl and ginsenoside Rb1 were 0.273 4-2.734 μg（r=0.999 9）, 0.119 1-1.191 μg（r=0.999 9）, 0.081 5-0.815 μg（r=0.999 9）, 0.622 8-6.228 μg（r=0.999 9）,0.807 2-8.072μg（r=0.999 9）, 1.036 4-10.364 μg（r=0.999 9）. The limits of quantitation were 0.082 0, 0.029 8, 0.028 5, 0.436 0, 0.403 6, 0.310 9μg. The limits of detection were 0.027 9, 0.009 5, 0.010 2, 0.124 6, 0.121 1,0.093 3 μg. RSDs of precision, stability and reproducibility tests were lower than 2.0%. Recoveries were 97.85%-100.34% （RSD=0.81%,n=6）, 98.14% -101.22% （RSD=1.09% , n=6） , 98.42% -102.15% （RSD=1.29% , n=6） , 97.77% -100.25% （RSD=0.96% , n=6） , 97.32%-99.53 % （RSD=0.81%, n=6） and 98.28%-101.51% （RSD= 1.11%, n=6）. CONCLUSIONS： The method is simple, rapid, stable, feasible and reproducible, and can be used for the simultaneous determination 6 components in Quyu zhitong mixture.

关 键 词：高效液相色谱法 祛瘀止痛合剂 芍药苷 柚皮苷 新橙皮苷 三七皂苷R1 人参皂苷RG1 人参皂苷RB1 

分 类 号：R917[医药卫生—药物分析学]