QuEChERS-超高效液相色谱-串联质谱法同时测定樱桃中的7种植物生长调节剂  

Simultaneous determination of QuEChERS-UPLC-MS on seven plant growth regulators in cherry

作　　者：魏玉霞[1] 任武洁 郭虹[1] 杨玉竹[1] 雷凯[1] 王建国[1] 王芳[1] 

机构地区：[1]北京市通州区疾病预防控制中心,北京101100

出　　处：《现代预防医学》2017年第12期2245-2250,共6页Modern Preventive Medicine

摘　　要：目的建立樱桃中7种植物生长调节剂(氯吡脲、赤霉素、噻苯隆、多效唑、4-氯苯氧乙酸、2,4-二氯苯氧乙酸和烯效唑)残留的Qu ECh ERS-超高效液相色谱-串联质谱技术同时测定方法。方法样品匀浆后经1%乙酸乙腈提取,分散固相萃取净化,以乙腈和0.01%氨水溶液为流动相进行梯度洗脱,ACQUITY BEH C18色谱柱分离,采用电喷雾多反应监测模式,正、负离子切换,外标法定量。结果 7种植物生长调节剂线性相关系数均>0.99,方法检出限在0.005~1.0μg/kg之间,定量限在0.015~3.0μg/kg之间,7种植物生长调节剂的平均回收率在74.0~101%之间,相对标准偏差小于5%。结论该方法简便、准确、灵敏、重现性好,可应用于樱桃样品的检测。Objective The study aimed to develop a method for determination of seven plant growth regulatorsinclndingforchlorfennron, gibberellin, thidiazuron, paclobutrazol, 4-chlorophenoxy acetic acid,2,4-dichlorophenoxy aceticacid, and unieonazole in cherry by QnEChERS-UPLC-MS. Methods After homogenization, sample was extracted withacetonitrile containing 1% acetic acid. The extracts were cleaned-up with dispersive-solid phase extraction.The targetchemicals were separated with ACQUITY BEH C18 column with gradient elution using acetonitrile and 0.01% ammoniaaqueous as mobile phases. Identification and determination were achieved by using multiple reaction monitoring mode,positive and negative ion switch. The quantification was performed using external standard method. Results The linearcorrelation coefficients of seven plant growth regulators were all above 0.99, detection limit of the method was 0.005-1.0tzg/kg, the quantitative limit was between 0.0151μg/kg and 3.0μg/kg, the average recovery rate ofseven plant growth regulatorsranged between 74.0% and 101%, and the relative standard deviation was less than 5%. Conclusion The method is simple,accurate,sensitive, and high in reproducibility, and it is suitable for the determination of plant growth regulators residues incherry

关 键 词：植物生长调节剂 QUECHERS 超高效液相色谱-串联质谱法 樱桃 

分 类 号：R115[医药卫生—公共卫生与预防医学]