Gab1和Sfmbt2基因在成年普通牛8种不同组织中的印记状态分析  

Genomic Imprinting Status of Gab1 and Sfmbt2 in Different Tissues of Adult Cattle

作　　者：王冠楠[1] 赵宇鹏[2] 陈玮娜[3] 张萃[1] 徐达[1] 李冬杰[4] 李世杰[1] 

机构地区：[1]河北农业大学生命科学学院,保定071001 [2]河北北方学院,张家口075000 [3]河北大学中医系,保定071001 [4]河北科技大学生物科学与工程学院,石家庄050018

出　　处：《畜牧兽医学报》2017年第6期1000-1006,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31372312);河北省自然科学基金(C2016204092)

摘　　要：旨在揭示Gab1(Grb2-associated binding protein 1)和Sfmbt2(Scm-like with four mbt domains 2)基因在牛不同组织以及胎盘中的印记状态,本研究应用基于单核苷酸多态性(Single nucleotide polymorphisms,SNP)的RT-PCR(Reverse transcription-PCR)产物直接测序方法对Gab1和Sfmbt2基因在牛7个组织(心、肝、脾、肺、肾、肌肉和脂肪)以及胎盘中的印记状态进行分析。结果显示,在被分析的7个组织和胎盘中均检测到Gab1和Sfmbt2基因的表达。通过比较杂合子牛基因组PCR扩增产物与RT-PCR扩增产物在SNP位点的测序峰图,发现Gab1和Sfmbt2在被检测的7个组织和胎盘中均为双等位基因表达,说明Gab1和Sfmbt2在牛的组织和胎盘中是非印记的。In order to reveal the imprinting status of Gab1 （Grb2-associated binding protein 1） and Sfmbt2 （Scm-like with four mbt domains 2） in different tissues and placenta of bovine, in this study, we analyzed the imprinting status of Gab1 and Sfmbt2 in 7 bovine tissues （heart, liver, spleen, lung, kidney, muscle and fat） and placenta by direct sequencing the RT-PCR products based on single nucleotide polymorphisms. The result displayed that Gab1 and Sfmbt2 were all expressed in 7 tissues and placenta. We discovered that Gab1 and Sfmbt2 were bi-allelic expressed by comparing sequence chromatograms of RT-PCR products and genomic PCR products at single nucleotide polymorphisms sites, suggesting that Gab1 and Sfmbt2 were not imprinted in adult cattle tissues and placenta.

关 键 词：Gab1 Sfmbt2 印记状态 SNP 牛 

分 类 号：S823[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]