黄芪、枸杞、金银花等中草药复方制剂对肉鸡免疫器官发育及免疫功能的影响  

The Effect of Compound Chinese Herbal Medicine Preparation of Astragalus,Wolfberry and Honeysuckle Flower on Immune Organ Development and Immune Functions of Broiler

作　　者：靳二辉[1] 陈耀星[2] 周金星[1] 李升和[1] 任曼[1] 胡倩倩[1] 金光明[1] 

机构地区：[1]安徽科技学院动物科学学院,凤阳233100 [2]中国农业大学动物医学院,北京100193

出　　处：《畜牧兽医学报》2017年第6期1128-1139,共12页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31402154);国家星火计划面上项目(2015GA710015);北京农学院兽医学(中兽医)北京市重点实验室开放课题(kf2016035);安徽省农业科技成果转化项目(1504032001);安徽省科技攻关项目(1301042102);安徽省高等学校自然科学研究重点项目(KJ2016A823;KJ2017A505);安徽省第七批"115"产业创新团队项目(皖人才[2014]4号);安徽科技学院重点建设学科项目(AKZDXK2015A04)

摘　　要：为了研究由黄芪、枸杞、金银花等药材组成的复方中草药制剂对肉鸡免疫器官发育及免疫功能的影响,将1日龄健康AA肉鸡1 000羽随机分为对照组和试验Ⅰ、Ⅱ、Ⅲ组,每组5个重复,每个重复50羽,对照组饲喂基础日粮,试验Ⅰ、Ⅱ和Ⅲ组分别在基础日粮中添加0.5%、1.0%和1.5%的复方中草药制剂,试验期42d。结果显示,在21日龄时,试验Ⅱ组肉鸡法氏囊指数、血清IgG和IgM含量以及NDV抗体和IL-4水平均显著高于对照组(P<0.05),试验Ⅲ组脾质量和器官指数以及血清IL-2和IFN-γ水平均显著高于对照组(P<0.05),试验Ⅰ组肉鸡血清IgG含量和NDV抗体水平均显著高于对照组(P<0.05);在42日龄时,试验Ⅱ组肉鸡胸腺和法氏囊质量与器官指数、血清IgG和IgM含量以及NDV抗体和IL-4水平均显著高于对照组(P<0.05),试验Ⅲ组肉鸡脾质量与器官指数、血清IgG和IgM含量以及IL-2、IL-4和IFN-γ水平均显著高于对照组(P<0.05),试验Ⅰ组肉鸡血清IgG和IgM含量以及NDV抗体水平均显著高于对照组(P<0.05)。显微观察可见,在21日龄时,试验Ⅱ组肉鸡法氏囊组织结构清晰,法氏囊小结面积显著大于对照组(P<0.05),小结内淋巴细胞数量较多,排列紧密。在42日龄时,试验Ⅱ组肉鸡胸腺皮质厚度显著大于对照组(P<0.05),法氏囊小结面积显著大于对照组(P<0.05);试验Ⅲ组肉鸡胸腺皮质厚度显著大于对照组(P<0.05),脾小结和脾动脉周围淋巴鞘小结面积显著大于对照组(P<0.05),脾小结数量也明显较多;试验Ⅰ组肉鸡脾小结面积也明显大于对照组(P<0.05)。结果表明,日粮中添加不同剂量黄芪、枸杞、金银花等复方中草药制剂对肉鸡免疫器官发育和免疫功能可产生不同程度的促进作用,其中以添加1.0%复方中草药制剂效果最好。To study the effect of compound Chinese herbal composed of Astragalus, Chinese wolf- berry, honeysuckle and other herbs on immune organ development and immune function of broil- ers. A total of 1 000 one-day-old Avian broilers （AA broilers） were randomly divided into control group and experimental groups Ⅰ , Ⅱand Ⅲ , each group included 5 pens （50 birds per pen）.Control group was fed with the basal diet and experimental groups Ⅰ , Ⅱ and Ⅲ were fed with the basal diet supplemented with 0.5%, 1.0% and 1.5% compound Chinese herbal for 42 days, re- spectively. Results showed, compared with control group, the index of bursa of Fabricius, serum IgG and IgM concentration, serum NDV antibody and IL-4 levels were significantly increased in experimental group Ⅱ （P〈0.05） ; the weight and organ index of thymus, the levels of serum IL- 2 and IFN-γ were significantly increased in experimental group Ⅲ （P〈0.05） ; the serum IgG con- centration and NDV antibody level were significantly increased in experimental group Ⅰ of broilers （P〈0.05）. At 42 days of age, the weight and organ index of thymus and bursa, the serum IgG and IgM concentration, the serum NDV antibody and IL-4 levels were significantly increased in experimental group Ⅱ （P〈0.05） ; the weight and organ index of spleen, the levels of serum IL- 2,IL-4 and IFN-γ, were significantly increased in experimental group Ⅲ （P〈0.05）; the serum IgG and IgM concentration, the serum NDV antibody level were significantly increased in experi- mental group I compared with control group of broilers （P〈0.05）. Microscopic observation has also showed. At 21 days of age, the structure of bursa of Fabricius was clear, the area of bursa of Fabricius node was obviously increased （P〈0.05） , in which the numbers of lymphocytes were also increased and tight arranged in experimental group II of broilers. At 42 days of age, the thy- mic cortex were thicker （P〈0.05） , and the area of bursa of Fabricius node was obviously

关 键 词：复方中草药 肉鸡 免疫器官 免疫功能 

分 类 号：S853.7[农业科学—临床兽医学]