槲皮素在聚离子液体膜修饰电极上的电化学行为及其测定  

Electrochemical behaviors and determination of quercetin at a polymerized ionic liquid film modified electrode

作　　者：瞿万云[1] 杨春海[1] 谭运斌 王鹏[1] 

机构地区：[1]湖北民族学院化学与环境工程学院,恩施445000

出　　处：《分析试验室》2017年第6期627-631,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(21465009)资助

摘　　要：合成了1-[3'-(N-吡咯)丙基]-3-己基咪唑四氟硼酸盐离子液体,以其为单体,采用循环伏安法(CV)制备出聚离子液体膜修饰玻碳电极,经十二烷基硫酸钠溶液处理实现阴离子交换,获得聚离子液体疏水膜界面(PIL/GCE),利用扫描电子显微镜(SEM)和K_3Fe(CN)_6/K_4Fe(CN)_6探针表征了该修饰电极的表面形貌和电化学性能,通过伏安法研究了槲皮素在PIL/GCE电极界面上的电化学行为。结果发现:槲皮素在该修饰电极上只有一个不可逆的氧化峰,与裸玻碳电极相比,氧化峰电流显著增强。优化了实验条件如:聚合膜的厚度,pH,富集电位和富集时间等。在优化条件下,槲皮素的氧化峰电流与浓度在0.5~3.0μmol/L和3.0~20μmol/L范围类有良好的线性关系,检测限为0.2μmol/L。方法已用于中药中槲皮素的测定。An ionic liquid 1-[3＇-（N-Pyrrolyl） propyl]-3-hexylimidazolium tetrafluoroborate was synthesized and used as a monomer to fabricate a polymerized ionic liquid film modified glassy carbon electrode with cyclic vohammetry. Followed by being treated with sodium dodecyl sulfate solution, a Poly [1 - [ 3- （N-Pyrrolyl） propyl ] -3-hexylimidazoliumdodecyl sulfonate ionic liquid t hydrophobic film modified electrode （PIL/GCE） was obtained. The morphology and electrochemical properties of the obtained polymerized ionic liquid film electrode were characterized with scanning electron microscope and using K3Fe（CN） 6/K4Fe（CN） 6 as eleetroactive probes. Electrochemical behaviors of quercetin at the polymerized ionic liquid film modified electrode were investigated with voltammetry. Only one oxidation peak was observed for quercetin at PIL/GCE , indicating an irreversible electrochemical oxidation process. Compared with the unmodified glassy carbon electrode, the oxidation peak current increased significantly. Experimental conditions such as film thickness, pH value, accumulation potential and time were optimized, respectively. Under optimal conditions, the oxidation peak current was linearly related to the concentration of quercetin in the range of 0. 5 - 3.0 μmol/L and 3.0 - 20 μmol/L with a limit of detection of 2.0 × 10 ^-7 mol/L. The proposed method was successfully used to determine quercetin in Chinese traditional medicine.

关 键 词：聚离子液体 吡咯 电分析 槲皮素 

分 类 号：R284[医药卫生—中药学] O657.1[医药卫生—中医学]